![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/Elyra-PS1-1024x1024.jpg\")
<\/figure><\/div>\n\n\n\nLasers<\/h2>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/11\/Elyra_311-confocal_spectrum-1024x212.png\")
<\/figure>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/11\/Elyra_311-SIM_STORM_spectrum-1024x212.png\")
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.3<\/td> 5.2<\/td> dry<\/td> Plan NeoFluar<\/td><\/tr> 63x<\/td> 1.4<\/td> 0.4<\/td> oil<\/td> Plan Apochromat DIC<\/td><\/tr> 100x<\/td> 1.46<\/td> 0.11<\/td> oil<\/td> \u03b1 Plan Apochromat DIC<\/td><\/tr> 100x<\/td> 1.57<\/td> 0.11<\/td> oil<\/td> Korr \u03b1 Plan Apochromat<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
Confocal<\/strong>
32 channel GaAsP (spectral range 412nm \u2013 691nm) (Gallium Arsenide Phosphide detector)
Blue PMT (spectral range 371nm \u2013 740nm) (Photo Multiplier Tube)
Red PMT (spectral range 379nm \u2013 758nm) (Photo Multiplier Tube)<\/p>\n\n\n\nSIM<\/strong>
EMCCD camera Andor iXon DU 885, 1002×1004 pixels<\/p>\n\n\n\nSingle Molecule imaging<\/strong>
EMCCD camera Andor iXon DU 897, 512x512pixels<\/p>\n\n\n\nFilters SIM\/PALM<\/h2>\n\n\n\n
Fset 77 HE (3 colour triple filter green, red, far red) only filter for visual inspection
BP 420-480 + LP 750, blue dyes
BP 495-575 + LP 750, green dyes
BP 570-650 + LP 750, red dyes
LP 655 , far red dyes<\/p>\n\n\n\n
Lasers<\/h2>\n\n\n\n
Confocal<\/strong>
405 nm – 30mW
458 nm, 488 nm, 514 nm – Argon
561 nm – 10mW
633 nm – HeNe<\/p>\n\n\n\nSIM\/PALM<\/strong>
405 nm – 50mW
488 nm – 100mW
561 nm – 100mW
642 nm – 100mW<\/p>\n\n\n\nExtra info<\/h2>\n\n\n\n
Mercury lamp for EPI fluorescence: Excelitas X-CITE\u00ae 120
Piezo stage
Tokai Hit lightweight stage top incubator for CO2<\/sub> and 37o<\/sup>C conditions with inserts for slides and 35mm dishes with feedback sensor, humidified CO2 <\/sub>controller and objective heating.
Zeiss Zen blue SIM reconstruction module
Zeiss Zen blue Single molecule analysis module\u00a0 <\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
Structured Illumination Microscopy (SIM)
Stochastic Optical Reconstruction Microscopy (STORM)
Photo Activation Localization Microscopy (PALM)
Single molecule localization microscopy (SMLM)<\/p>\n\n\n\n
Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions FRAP, FRET, Time lapse imaging
Fixed cell imaging<\/p>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"Zeiss Elyra PS1 SIM, PALM modules and LSM 780 confocal Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.3 5.2 dry Plan NeoFluar 63x 1.4 0.4 oil Plan Apochromat DIC 100x 1.46 0.11 oil \u03b1 Plan Apochromat DIC 100x 1.57 0.11 oil Korr \u03b1 Plan Apochromat Detectors Confocal32 channel GaAsP (spectral range 412nm \u2013 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1370","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Zeiss Elyra PS1 SIM, PALM modules and LSM 780 confocal Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.3 5.2 dry Plan NeoFluar 63x 1.4 0.4 oil Plan Apochromat DIC 100x 1.46 0.11 oil \u03b1 Plan Apochromat DIC 100x 1.57 0.11 oil Korr \u03b1 Plan Apochromat Detectors Confocal32 channel GaAsP (spectral range 412nm \u2013…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1370"}],"version-history":[{"count":12,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370\/revisions"}],"predecessor-version":[{"id":3271,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370\/revisions\/3271"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1370"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>\n\n\n\n<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.3<\/td> 5.2<\/td> dry<\/td> Plan NeoFluar<\/td><\/tr> 63x<\/td> 1.4<\/td> 0.4<\/td> oil<\/td> Plan Apochromat DIC<\/td><\/tr> 100x<\/td> 1.46<\/td> 0.11<\/td> oil<\/td> \u03b1 Plan Apochromat DIC<\/td><\/tr> 100x<\/td> 1.57<\/td> 0.11<\/td> oil<\/td> Korr \u03b1 Plan Apochromat<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
Confocal<\/strong>
32 channel GaAsP (spectral range 412nm \u2013 691nm) (Gallium Arsenide Phosphide detector)
Blue PMT (spectral range 371nm \u2013 740nm) (Photo Multiplier Tube)
Red PMT (spectral range 379nm \u2013 758nm) (Photo Multiplier Tube)<\/p>\n\n\n\nSIM<\/strong>
EMCCD camera Andor iXon DU 885, 1002×1004 pixels<\/p>\n\n\n\nSingle Molecule imaging<\/strong>
EMCCD camera Andor iXon DU 897, 512x512pixels<\/p>\n\n\n\nFilters SIM\/PALM<\/h2>\n\n\n\n
Fset 77 HE (3 colour triple filter green, red, far red) only filter for visual inspection
BP 420-480 + LP 750, blue dyes
BP 495-575 + LP 750, green dyes
BP 570-650 + LP 750, red dyes
LP 655 , far red dyes<\/p>\n\n\n\n
Lasers<\/h2>\n\n\n\n
Confocal<\/strong>
405 nm – 30mW
458 nm, 488 nm, 514 nm – Argon
561 nm – 10mW
633 nm – HeNe<\/p>\n\n\n\nSIM\/PALM<\/strong>
405 nm – 50mW
488 nm – 100mW
561 nm – 100mW
642 nm – 100mW<\/p>\n\n\n\nExtra info<\/h2>\n\n\n\n
Mercury lamp for EPI fluorescence: Excelitas X-CITE\u00ae 120
Piezo stage
Tokai Hit lightweight stage top incubator for CO2<\/sub> and 37o<\/sup>C conditions with inserts for slides and 35mm dishes with feedback sensor, humidified CO2 <\/sub>controller and objective heating.
Zeiss Zen blue SIM reconstruction module
Zeiss Zen blue Single molecule analysis module\u00a0 <\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
Structured Illumination Microscopy (SIM)
Stochastic Optical Reconstruction Microscopy (STORM)
Photo Activation Localization Microscopy (PALM)
Single molecule localization microscopy (SMLM)<\/p>\n\n\n\n
Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions FRAP, FRET, Time lapse imaging
Fixed cell imaging<\/p>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"Zeiss Elyra PS1 SIM, PALM modules and LSM 780 confocal Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.3 5.2 dry Plan NeoFluar 63x 1.4 0.4 oil Plan Apochromat DIC 100x 1.46 0.11 oil \u03b1 Plan Apochromat DIC 100x 1.57 0.11 oil Korr \u03b1 Plan Apochromat Detectors Confocal32 channel GaAsP (spectral range 412nm \u2013 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1370","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Zeiss Elyra PS1 SIM, PALM modules and LSM 780 confocal Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.3 5.2 dry Plan NeoFluar 63x 1.4 0.4 oil Plan Apochromat DIC 100x 1.46 0.11 oil \u03b1 Plan Apochromat DIC 100x 1.57 0.11 oil Korr \u03b1 Plan Apochromat Detectors Confocal32 channel GaAsP (spectral range 412nm \u2013…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1370"}],"version-history":[{"count":12,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370\/revisions"}],"predecessor-version":[{"id":3271,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370\/revisions\/3271"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1370"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Magnification<\/strong><\/td>| Numerical | Aperture<\/strong><\/td> Working | Distance (mm)<\/strong><\/td> Immersion<\/strong><\/td> | Type<\/strong><\/td><\/tr> | 10x<\/td> | 0.3<\/td> | 5.2<\/td> | dry<\/td> | Plan NeoFluar<\/td><\/tr> | 63x<\/td> | 1.4<\/td> | 0.4<\/td> | oil<\/td> | Plan Apochromat DIC<\/td><\/tr> | 100x<\/td> | 1.46<\/td> | 0.11<\/td> | oil<\/td> | \u03b1 Plan Apochromat DIC<\/td><\/tr> | 100x<\/td> | 1.57<\/td> | 0.11<\/td> | oil<\/td> | Korr \u03b1 Plan Apochromat<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n | Detectors<\/h2>\n\n\n\nConfocal<\/strong> SIM<\/strong> Single Molecule imaging<\/strong> Fset 77 HE (3 colour triple filter green, red, far red) only filter for visual inspection Confocal<\/strong> SIM\/PALM<\/strong> Mercury lamp for EPI fluorescence: Excelitas X-CITE\u00ae 120 Structured Illumination Microscopy (SIM) Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions FRAP, FRET, Time lapse imaging Zeiss Elyra PS1 SIM, PALM modules and LSM 780 confocal Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.3 5.2 dry Plan NeoFluar 63x 1.4 0.4 oil Plan Apochromat DIC 100x 1.46 0.11 oil \u03b1 Plan Apochromat DIC 100x 1.57 0.11 oil Korr \u03b1 Plan Apochromat Detectors Confocal32 channel GaAsP (spectral range 412nm \u2013 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1370","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Zeiss Elyra PS1 SIM, PALM modules and LSM 780 confocal Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.3 5.2 dry Plan NeoFluar 63x 1.4 0.4 oil Plan Apochromat DIC 100x 1.46 0.11 oil \u03b1 Plan Apochromat DIC 100x 1.57 0.11 oil Korr \u03b1 Plan Apochromat Detectors Confocal32 channel GaAsP (spectral range 412nm \u2013…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1370"}],"version-history":[{"count":12,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370\/revisions"}],"predecessor-version":[{"id":3271,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1370\/revisions\/3271"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1370"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}} |