![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/Nikon-spin-D.jpg\")
<\/figure>\n<\/div>\n\n\nLasers<\/h2>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/Nikon-spin-D-spectrum-1024x219.png\")
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Pixel size (nm<\/strong>)<\/td>Numerical<\/strong>
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>20x<\/td> 297<\/td> 0.75<\/td> 1.00<\/td> dry<\/td> Plan APO DIC<\/td><\/tr> 40x<\/td> 169<\/td> 1.30<\/td> 0.20<\/td> oil<\/td> Plan APO DIC<\/td><\/tr> 63x<\/td> 115<\/td> 1.40<\/td> 0.13<\/td> oil<\/td> Pan APO VC<\/td><\/tr> 100x<\/td> 69<\/td> 1.49<\/td> 0.12<\/td> oil<\/td> Plan APO TIRF<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nCamera<\/h2>\n\n\n\n
EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)<\/p>\n\n\n\n
Filters<\/h2>\n\n\n\nColour<\/strong><\/td>Exitation<\/strong><\/td>Emission<\/strong><\/td>Type<\/strong><\/td><\/tr>Blue<\/td> 350\/50x<\/td> 460\/40m<\/td> Chroma 49000 ET-DAPI<\/td><\/tr> Green<\/td> 470\/40x<\/td> 525\/50m<\/td> Chroma 49002 ET-GFP<\/td><\/tr> Red<\/td> 560\/40x<\/td> 630\/75m<\/td> Chroma 49008 ET-mCh\/tR<\/td><\/tr> Green + Red<\/td> 450-490 + 550-590<\/td> 525\/25m + 630\/30m<\/td> Chroma 59022 ET-GFP\/mCherry<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nExtra info<\/h2>\n\n\n\n
Imaging under CO2<\/sub> and 30\/37o<\/sup>C conditions, with stage top incubator, CO2<\/sub> controller, objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
Spinning Disc unit, CSU-X1 (Yokogawa)
FRAP3D unit: ultrafast by Galvo mirror positioned laserbleaching (ROPER)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green\/Red or Cyan\/Green
Pi\u00ebzo stage for fast Z-stacks
Hardware autofocus: Perfect Focus System (Nikon)
Metamorph Imaging Software<\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
Fast Live cell confocal like imaging, with less monitor bleaching (in vitro or in vivo)
Live Cell imaging imaging conditions.
Multichannel 4D imaging (3D timelapse imaging)
Imaging activatable or switchable fluorophores
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Fixed cell imaging<\/p>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"Nikon Ti-Eclipse inverted microscope with Spinning disc unit and QuantEM EMCCD camera Lasers Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 20x 297 0.75 1.00 dry Plan APO DIC 40x 169 1.30 0.20 oil Plan APO DIC 63x 115 1.40 0.13 oil Pan APO VC 100x 69 1.49 0.12 oil Plan APO TIRF […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1400","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Nikon Ti-Eclipse inverted microscope with Spinning disc unit and QuantEM EMCCD camera Lasers Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 20x 297 0.75 1.00 dry Plan APO DIC 40x 169 1.30 0.20 oil Plan APO DIC 63x 115 1.40 0.13 oil Pan APO VC 100x 69 1.49 0.12 oil Plan APO TIRF…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1400","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1400"}],"version-history":[{"count":14,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1400\/revisions"}],"predecessor-version":[{"id":4721,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1400\/revisions\/4721"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1400"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Pixel size (nm<\/strong>)<\/td>Numerical<\/strong>
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>20x<\/td> 297<\/td> 0.75<\/td> 1.00<\/td> dry<\/td> Plan APO DIC<\/td><\/tr> 40x<\/td> 169<\/td> 1.30<\/td> 0.20<\/td> oil<\/td> Plan APO DIC<\/td><\/tr> 63x<\/td> 115<\/td> 1.40<\/td> 0.13<\/td> oil<\/td> Pan APO VC<\/td><\/tr> 100x<\/td> 69<\/td> 1.49<\/td> 0.12<\/td> oil<\/td> Plan APO TIRF<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nCamera<\/h2>\n\n\n\n
EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)<\/p>\n\n\n\n
Filters<\/h2>\n\n\n\nColour<\/strong><\/td>Exitation<\/strong><\/td>Emission<\/strong><\/td>Type<\/strong><\/td><\/tr>Blue<\/td> 350\/50x<\/td> 460\/40m<\/td> Chroma 49000 ET-DAPI<\/td><\/tr> Green<\/td> 470\/40x<\/td> 525\/50m<\/td> Chroma 49002 ET-GFP<\/td><\/tr> Red<\/td> 560\/40x<\/td> 630\/75m<\/td> Chroma 49008 ET-mCh\/tR<\/td><\/tr> Green + Red<\/td> 450-490 + 550-590<\/td> 525\/25m + 630\/30m<\/td> Chroma 59022 ET-GFP\/mCherry<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nExtra info<\/h2>\n\n\n\n
Imaging under CO2<\/sub> and 30\/37o<\/sup>C conditions, with stage top incubator, CO2<\/sub> controller, objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
Spinning Disc unit, CSU-X1 (Yokogawa)
FRAP3D unit: ultrafast by Galvo mirror positioned laserbleaching (ROPER)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green\/Red or Cyan\/Green
Pi\u00ebzo stage for fast Z-stacks
Hardware autofocus: Perfect Focus System (Nikon)
Metamorph Imaging Software<\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
Fast Live cell confocal like imaging, with less monitor bleaching (in vitro or in vivo)
Live Cell imaging imaging conditions.
Multichannel 4D imaging (3D timelapse imaging)
Imaging activatable or switchable fluorophores
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Fixed cell imaging<\/p>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"Nikon Ti-Eclipse inverted microscope with Spinning disc unit and QuantEM EMCCD camera Lasers Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 20x 297 0.75 1.00 dry Plan APO DIC 40x 169 1.30 0.20 oil Plan APO DIC 63x 115 1.40 0.13 oil Pan APO VC 100x 69 1.49 0.12 oil Plan APO TIRF […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1400","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Nikon Ti-Eclipse inverted microscope with Spinning disc unit and QuantEM EMCCD camera Lasers Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 20x 297 0.75 1.00 dry Plan APO DIC 40x 169 1.30 0.20 oil Plan APO DIC 63x 115 1.40 0.13 oil Pan APO VC 100x 69 1.49 0.12 oil Plan APO TIRF…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1400","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1400"}],"version-history":[{"count":14,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1400\/revisions"}],"predecessor-version":[{"id":4721,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1400\/revisions\/4721"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1400"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Magnification<\/strong><\/td>| Pixel size (nm<\/strong>)<\/td> | Numerical<\/strong> | Aperture<\/strong><\/td> Working | Distance (mm)<\/strong><\/td> Immersion<\/strong><\/td> | Type<\/strong><\/td><\/tr> | 20x<\/td> | 297<\/td> | 0.75<\/td> | 1.00<\/td> | dry<\/td> | Plan APO DIC<\/td><\/tr> | 40x<\/td> | 169<\/td> | 1.30<\/td> | 0.20<\/td> | oil<\/td> | Plan APO DIC<\/td><\/tr> | 63x<\/td> | 115<\/td> | 1.40<\/td> | 0.13<\/td> | oil<\/td> | Pan APO VC<\/td><\/tr> | 100x<\/td> | 69<\/td> | 1.49<\/td> | 0.12<\/td> | oil<\/td> | Plan APO TIRF<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n | Camera<\/h2>\n\n\n\nEM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)<\/p>\n\n\n\n Filters<\/h2>\n\n\n\n
|