![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/Nikon-TIRF.jpg\")
<\/figure>\n<\/div>\n\n\nLasers<\/h2>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/Nikon-TIRF-spectrum-1024x219.png\")
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Pixel size (nm<\/strong>)<\/td>Numerical<\/strong>
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 430<\/td> 0.45<\/td> 4.00<\/td> dry<\/td> Plan APO DIC<\/td><\/tr> 60x<\/td> 111<\/td> 1.45<\/td> 0.13<\/td> oil<\/td> Pan APO TIRF<\/td><\/tr> 100x<\/td> 66<\/td> 1.49<\/td> 0.12<\/td> oil<\/td> Plan APO TIRF<\/td><\/tr> 100x<\/td> 66<\/td> 1.40<\/td> 0.13<\/td> oil<\/td> Plan APO VC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nCamera<\/h2>\n\n\n\n
EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)<\/p>\n\n\n\n
Filters<\/h2>\n\n\n\nColour<\/strong><\/td>Exitation<\/strong><\/td>Emission<\/strong><\/td>Type<\/strong><\/td><\/tr>Blue<\/td> 350\/50x<\/td> 460\/40m<\/td> Chroma 49000 ET-DAPI<\/td><\/tr> Green<\/td> 470\/40x<\/td> 525\/50m<\/td> Chroma 49002 ET-GFP<\/td><\/tr> Red<\/td> 560\/40x<\/td> 630\/75m<\/td> Chroma 49008 ET-mCh\/tR<\/td><\/tr> Green \/ Red<\/td> 450-490 + 550-590<\/td> 525\/25m + 630\/30m<\/td> Chroma 59022 ET-GFP\/mCherry<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nExtra info<\/h2>\n\n\n\n
Imaging under 5% CO2<\/sub> and 30\/37o<\/sup>C conditions, with stage top incubator, CO2<\/sub> controller and objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
TIRF mount for manual or mechanic TIRF angle control
FRAP3D unit: ultrafast by galvo mirror positioned laserbleaching (ROPER)
Hardware autofocus: Perfect Focus System (Nikon)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green\/Red or Cyan\/Green
Metamorph Imaging Software<\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
Live cell TIRF\/WideField imaging conditions.
Membrane studies (in vitro & in vivo)
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Single Molecule Imaging, PALM and STORM
Imaging activatable or switchable fluorophores
Fixed cell imaging<\/p>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"Nikon Ti-Eclipse inverted microscope with TIRF unit and QuantEM EMCCD camera Lasers Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 10x 430 0.45 4.00 dry Plan APO DIC 60x 111 1.45 0.13 oil Pan APO TIRF 100x 66 1.49 0.12 oil Plan APO TIRF 100x 66 1.40 0.13 oil Plan APO VC Camera […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1406","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Nikon Ti-Eclipse inverted microscope with TIRF unit and QuantEM EMCCD camera Lasers Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 10x 430 0.45 4.00 dry Plan APO DIC 60x 111 1.45 0.13 oil Pan APO TIRF 100x 66 1.49 0.12 oil Plan APO TIRF 100x 66 1.40 0.13 oil Plan APO VC Camera…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1406","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1406"}],"version-history":[{"count":13,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1406\/revisions"}],"predecessor-version":[{"id":4724,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1406\/revisions\/4724"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1406"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Pixel size (nm<\/strong>)<\/td>Numerical<\/strong>
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 430<\/td> 0.45<\/td> 4.00<\/td> dry<\/td> Plan APO DIC<\/td><\/tr> 60x<\/td> 111<\/td> 1.45<\/td> 0.13<\/td> oil<\/td> Pan APO TIRF<\/td><\/tr> 100x<\/td> 66<\/td> 1.49<\/td> 0.12<\/td> oil<\/td> Plan APO TIRF<\/td><\/tr> 100x<\/td> 66<\/td> 1.40<\/td> 0.13<\/td> oil<\/td> Plan APO VC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nCamera<\/h2>\n\n\n\n
EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)<\/p>\n\n\n\n
Filters<\/h2>\n\n\n\nColour<\/strong><\/td>Exitation<\/strong><\/td>Emission<\/strong><\/td>Type<\/strong><\/td><\/tr>Blue<\/td> 350\/50x<\/td> 460\/40m<\/td> Chroma 49000 ET-DAPI<\/td><\/tr> Green<\/td> 470\/40x<\/td> 525\/50m<\/td> Chroma 49002 ET-GFP<\/td><\/tr> Red<\/td> 560\/40x<\/td> 630\/75m<\/td> Chroma 49008 ET-mCh\/tR<\/td><\/tr> Green \/ Red<\/td> 450-490 + 550-590<\/td> 525\/25m + 630\/30m<\/td> Chroma 59022 ET-GFP\/mCherry<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nExtra info<\/h2>\n\n\n\n
Imaging under 5% CO2<\/sub> and 30\/37o<\/sup>C conditions, with stage top incubator, CO2<\/sub> controller and objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
TIRF mount for manual or mechanic TIRF angle control
FRAP3D unit: ultrafast by galvo mirror positioned laserbleaching (ROPER)
Hardware autofocus: Perfect Focus System (Nikon)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green\/Red or Cyan\/Green
Metamorph Imaging Software<\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
Live cell TIRF\/WideField imaging conditions.
Membrane studies (in vitro & in vivo)
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Single Molecule Imaging, PALM and STORM
Imaging activatable or switchable fluorophores
Fixed cell imaging<\/p>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"Nikon Ti-Eclipse inverted microscope with TIRF unit and QuantEM EMCCD camera Lasers Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 10x 430 0.45 4.00 dry Plan APO DIC 60x 111 1.45 0.13 oil Pan APO TIRF 100x 66 1.49 0.12 oil Plan APO TIRF 100x 66 1.40 0.13 oil Plan APO VC Camera […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1406","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Nikon Ti-Eclipse inverted microscope with TIRF unit and QuantEM EMCCD camera Lasers Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 10x 430 0.45 4.00 dry Plan APO DIC 60x 111 1.45 0.13 oil Pan APO TIRF 100x 66 1.49 0.12 oil Plan APO TIRF 100x 66 1.40 0.13 oil Plan APO VC Camera…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1406","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1406"}],"version-history":[{"count":13,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1406\/revisions"}],"predecessor-version":[{"id":4724,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1406\/revisions\/4724"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1406"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Magnification<\/strong><\/td>| Pixel size (nm<\/strong>)<\/td> | Numerical<\/strong> | Aperture<\/strong><\/td> Working | Distance (mm)<\/strong><\/td> Immersion<\/strong><\/td> | Type<\/strong><\/td><\/tr> | 10x<\/td> | 430<\/td> | 0.45<\/td> | 4.00<\/td> | dry<\/td> | Plan APO DIC<\/td><\/tr> | 60x<\/td> | 111<\/td> | 1.45<\/td> | 0.13<\/td> | oil<\/td> | Pan APO TIRF<\/td><\/tr> | 100x<\/td> | 66<\/td> | 1.49<\/td> | 0.12<\/td> | oil<\/td> | Plan APO TIRF<\/td><\/tr> | 100x<\/td> | 66<\/td> | 1.40<\/td> | 0.13<\/td> | oil<\/td> | Plan APO VC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n | Camera<\/h2>\n\n\n\nEM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)<\/p>\n\n\n\n Filters<\/h2>\n\n\n\n
|