{"id":1411,"date":"2020-02-17T15:25:17","date_gmt":"2020-02-17T15:25:17","guid":{"rendered":"https:\/\/erasmusoic.nl\/2020\/?page_id=1411"},"modified":"2024-01-04T10:27:13","modified_gmt":"2024-01-04T10:27:13","slug":"nikon-widefield-1078-ee-1078","status":"publish","type":"page","link":"https:\/\/erasmusoic.nl\/2020\/facility\/equipment\/nikon-widefield-1078-ee-1078\/","title":{"rendered":"Nikon WideField 1078 \/ Ee-1078"},"content":{"rendered":"\n

Nikon WideField with Ti-Eclipse inverted microscope and Coolsnap CCD camera<\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

Spectrum<\/h2>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Objectives<\/h2>\n\n\n\n
Magnification<\/strong><\/td>Pixel size (nm<\/strong>)<\/td>Numerical<\/strong>
Aperture<\/strong><\/td>		Working
Distance (mm)<\/strong><\/td>		Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>
4x<\/td>1600<\/td>0.20<\/td>20.0<\/td>dry<\/td>Plan APO<\/td><\/tr>
10x<\/td>430<\/td>0.45<\/td>4.00<\/td>dry<\/td>Plan APO DIC<\/td><\/tr>
20x<\/td>323<\/td>0.75<\/td>1.00<\/td>dry<\/td>Plan APO<\/td><\/tr>
Optional<\/strong><\/td><\/td><\/td><\/td><\/td><\/td><\/tr>
40x<\/em><\/td>157<\/em><\/td>1.30<\/em><\/td>0.20<\/em><\/td>oil<\/em><\/td>Plan Fluor<\/em><\/td><\/tr>
60x<\/em><\/td>108<\/em><\/td>1.40<\/em><\/td>0.13<\/em><\/td>oil<\/em><\/td>Plan VC<\/em><\/td><\/tr>
100x<\/em><\/td>64<\/em><\/td>1.40<\/em><\/td>0.13<\/em><\/td>oil<\/em><\/td>Plan VC<\/em><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Camera<\/h2>\n\n\n\n

Cooled CCD camera: Coolsnap HQ2 1390x1040pixels 14bit (Photometrics)<\/p>\n\n\n\n

Filters<\/h2>\n\n\n\n
Colour<\/strong><\/td>Exitation<\/strong><\/td>Emission<\/strong><\/td>Type<\/strong><\/td><\/tr>
Blue<\/td>350\/50x<\/td>460\/40m<\/td>Chroma 49000 ET-DAPI<\/td><\/tr>
Green<\/td>470\/40x<\/td>525\/50m<\/td>Chroma 49002 ET-GFP<\/td><\/tr>
Yellow<\/td>500\/20x<\/td>535\/30m<\/td>Chroma 49003 ET-EYFP<\/td><\/tr>
Red<\/td>560\/40x<\/td>630\/75m<\/td>Chroma 49008 ET-mCh\/tR<\/td><\/tr>
Far Red<\/td>et640\/30x<\/td>et705\/72m<\/td>Customized Cy5 filter
with BS t660lpxr mirror<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Extra info<\/h2>\n\n\n\n

Mercury lamp with ND4, ND8 and complete block filter 
Imaging under 5% CO2<\/sub> and 37\u00b0C conditions, with stage top incubator, CO2<\/sub> controller and objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
Hardware autofocus: Perfect Focus System (Nikon)  <\/p>\n\n\n\n

Applications<\/h2>\n\n\n\n

Live cell WideField imaging under CO2<\/sub> and 37\u00b0C conditions 
(Long term) time lapse imaging
Fixed cell imaging  <\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Nikon WideField with Ti-Eclipse inverted microscope and Coolsnap CCD camera Spectrum Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 4x 1600 0.20 20.0 dry Plan APO 10x 430 0.45 4.00 dry Plan APO DIC 20x 323 0.75 1.00 dry Plan APO Optional 40x 157 1.30 0.20 oil Plan Fluor 60x 108 1.40 0.13 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1411","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Nikon WideField with Ti-Eclipse inverted microscope and Coolsnap CCD camera Spectrum Objectives Magnification Pixel size (nm) NumericalAperture WorkingDistance (mm) Immersion Type 4x 1600 0.20 20.0 dry Plan APO 10x 430 0.45 4.00 dry Plan APO DIC 20x 323 0.75 1.00 dry Plan APO Optional 40x 157 1.30 0.20 oil Plan Fluor 60x 108 1.40 0.13…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1411","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1411"}],"version-history":[{"count":13,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1411\/revisions"}],"predecessor-version":[{"id":3901,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1411\/revisions\/3901"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1411"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}