![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-778.jpg\")
<\/figure><\/div>\n\n\n\nLasers<\/h2>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-778-spectrum-1024x219.png\")
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.40<\/td> 2.2<\/td> dry<\/td> HCX PL APO CS<\/td><\/tr> 20x<\/td> 0.70<\/td> 0.59<\/td> dry<\/td> HCX PL APO CS<\/td><\/tr> 40x<\/td> 1.25<\/td> 0.10<\/td> oil<\/td> HCX PL APO CS<\/td><\/tr> 63x<\/td> 1.40<\/td> 0.14<\/td> oil<\/td> HCX PL APO CS<\/td><\/tr> 100x<\/td> 1.35<\/td> –<\/td> glycerol<\/td> –<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
3x spectral PMT detector (Photo Multiplier Tube)
2x spectral APD detector (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
Multiphoton laser Coherent Chameleon Ultra (710 – 1040nm)
266nm UV laser
Avalanche photodiodes for extremely low signals<\/p>\n\n\n\n
Leica LAS-AF software with:
FRAP & FRET modules
Live data mode
Matrix screening software for high content screening
Leica DMi6000 inverted microscope stand with:
Motorized stage with inserts for slides, 35mm dishes, and multiwell plates
Peecon small chamber incubator with heating, CO2<\/sub> and O2<\/sub> regulation
Metal-Halide lamp for EPI fluorescence
<\/p>\n\n\n\n<\/p>\n\n\n\n
Applications<\/h2>\n\n\n\n
This microscope is especially equipped for making localized DNA damage and\/or multiphoton imaging.
Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging<\/p>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"Leica SP5 AOBS Multi-photon lasers Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.2 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS 100x 1.35 – glycerol – Detectors 3x spectral PMT detector […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1422","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP5 AOBS Multi-photon lasers Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.2 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS 100x 1.35 – glycerol – Detectors 3x spectral PMT detector…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1422"}],"version-history":[{"count":10,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422\/revisions"}],"predecessor-version":[{"id":3132,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422\/revisions\/3132"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.40<\/td> 2.2<\/td> dry<\/td> HCX PL APO CS<\/td><\/tr> 20x<\/td> 0.70<\/td> 0.59<\/td> dry<\/td> HCX PL APO CS<\/td><\/tr> 40x<\/td> 1.25<\/td> 0.10<\/td> oil<\/td> HCX PL APO CS<\/td><\/tr> 63x<\/td> 1.40<\/td> 0.14<\/td> oil<\/td> HCX PL APO CS<\/td><\/tr> 100x<\/td> 1.35<\/td> –<\/td> glycerol<\/td> –<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
3x spectral PMT detector (Photo Multiplier Tube)
2x spectral APD detector (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
Multiphoton laser Coherent Chameleon Ultra (710 – 1040nm)
266nm UV laser
Avalanche photodiodes for extremely low signals<\/p>\n\n\n\n
Leica LAS-AF software with:
FRAP & FRET modules
Live data mode
Matrix screening software for high content screening
Leica DMi6000 inverted microscope stand with:
Motorized stage with inserts for slides, 35mm dishes, and multiwell plates
Peecon small chamber incubator with heating, CO2<\/sub> and O2<\/sub> regulation
Metal-Halide lamp for EPI fluorescence
<\/p>\n\n\n\n<\/p>\n\n\n\n
Applications<\/h2>\n\n\n\n
This microscope is especially equipped for making localized DNA damage and\/or multiphoton imaging.
Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging<\/p>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"Leica SP5 AOBS Multi-photon lasers Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.2 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS 100x 1.35 – glycerol – Detectors 3x spectral PMT detector […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1422","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP5 AOBS Multi-photon lasers Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.2 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS 100x 1.35 – glycerol – Detectors 3x spectral PMT detector…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1422"}],"version-history":[{"count":10,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422\/revisions"}],"predecessor-version":[{"id":3132,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422\/revisions\/3132"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Magnification<\/strong><\/td>| Numerical | Aperture<\/strong><\/td> Working | Distance (mm)<\/strong><\/td> Immersion<\/strong><\/td> | Type<\/strong><\/td><\/tr> | 10x<\/td> | 0.40<\/td> | 2.2<\/td> | dry<\/td> | HCX PL APO CS<\/td><\/tr> | 20x<\/td> | 0.70<\/td> | 0.59<\/td> | dry<\/td> | HCX PL APO CS<\/td><\/tr> | 40x<\/td> | 1.25<\/td> | 0.10<\/td> | oil<\/td> | HCX PL APO CS<\/td><\/tr> | 63x<\/td> | 1.40<\/td> | 0.14<\/td> | oil<\/td> | HCX PL APO CS<\/td><\/tr> | 100x<\/td> | 1.35<\/td> | –<\/td> | glycerol<\/td> | –<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n | Detectors<\/h2>\n\n\n\n3x spectral PMT detector (Photo Multiplier Tube) Extra info<\/h2>\n\n\n\nMultiphoton laser Coherent Chameleon Ultra (710 – 1040nm) Leica LAS-AF software with: <\/p>\n\n\n\n This microscope is especially equipped for making localized DNA damage and\/or multiphoton imaging. Leica SP5 AOBS Multi-photon lasers Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.2 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS 100x 1.35 – glycerol – Detectors 3x spectral PMT detector […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1422","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP5 AOBS Multi-photon lasers Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.2 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS 100x 1.35 – glycerol – Detectors 3x spectral PMT detector…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1422"}],"version-history":[{"count":10,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422\/revisions"}],"predecessor-version":[{"id":3132,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1422\/revisions\/3132"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}} |