![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2022\/03\/SP5_1315-edited-scaled.jpg\")
<\/figure><\/div>\n\n\n\nLasers<\/h2>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-1315-spectrum-1024x219.png\")
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.40<\/td> 2.20<\/td> dry<\/td> HCX PL APO CS<\/td><\/tr> 20x<\/td> 0.70<\/td> 0.59<\/td> dry<\/td> HCX PL APO CS<\/td><\/tr> 20x<\/td> 0.70<\/td> 0.25<\/td> water\/glyc.\/oil<\/td> HCX PL Fluotar L<\/td><\/tr> 40x<\/td> 1.25<\/td> 0.10<\/td> oil<\/td> HCX PL APO CS<\/td><\/tr> 63x<\/td> 1.40<\/td> 0.14<\/td> oil<\/td> HCX PL APO CS<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
3x spectral PMT detector (Photo Multiplier Tube)
2x spectral APD detector (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
Leica LAS-AF software with:
Matrix screening software for high content screening
FRAP & FRET modules<\/p>\n\n\n\n
Motorized stage with inserts for slides, 35mm dishes, and multiwell plates
Metal-Halide lamp for EPI fluorescence
Avalance photo diodes for very high sensitivity
Peecon small chamber incubator with heating, CO2<\/sub> and O2<\/sub> regulation
<\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging<\/p>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"Leica SP5 laser scanning confocal with AOBS Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 20x 0.70 0.25 water\/glyc.\/oil HCX PL Fluotar L 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1425","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP5 laser scanning confocal with AOBS Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 20x 0.70 0.25 water\/glyc.\/oil HCX PL Fluotar L 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1425"}],"version-history":[{"count":7,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425\/revisions"}],"predecessor-version":[{"id":3108,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425\/revisions\/3108"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.40<\/td> 2.20<\/td> dry<\/td> HCX PL APO CS<\/td><\/tr> 20x<\/td> 0.70<\/td> 0.59<\/td> dry<\/td> HCX PL APO CS<\/td><\/tr> 20x<\/td> 0.70<\/td> 0.25<\/td> water\/glyc.\/oil<\/td> HCX PL Fluotar L<\/td><\/tr> 40x<\/td> 1.25<\/td> 0.10<\/td> oil<\/td> HCX PL APO CS<\/td><\/tr> 63x<\/td> 1.40<\/td> 0.14<\/td> oil<\/td> HCX PL APO CS<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
3x spectral PMT detector (Photo Multiplier Tube)
2x spectral APD detector (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
Leica LAS-AF software with:
Matrix screening software for high content screening
FRAP & FRET modules<\/p>\n\n\n\n
Motorized stage with inserts for slides, 35mm dishes, and multiwell plates
Metal-Halide lamp for EPI fluorescence
Avalance photo diodes for very high sensitivity
Peecon small chamber incubator with heating, CO2<\/sub> and O2<\/sub> regulation
<\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging<\/p>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"Leica SP5 laser scanning confocal with AOBS Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 20x 0.70 0.25 water\/glyc.\/oil HCX PL Fluotar L 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1425","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP5 laser scanning confocal with AOBS Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 20x 0.70 0.25 water\/glyc.\/oil HCX PL Fluotar L 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1425"}],"version-history":[{"count":7,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425\/revisions"}],"predecessor-version":[{"id":3108,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425\/revisions\/3108"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Magnification<\/strong><\/td>| Numerical | Aperture<\/strong><\/td> Working | Distance (mm)<\/strong><\/td> Immersion<\/strong><\/td> | Type<\/strong><\/td><\/tr> | 10x<\/td> | 0.40<\/td> | 2.20<\/td> | dry<\/td> | HCX PL APO CS<\/td><\/tr> | 20x<\/td> | 0.70<\/td> | 0.59<\/td> | dry<\/td> | HCX PL APO CS<\/td><\/tr> | 20x<\/td> | 0.70<\/td> | 0.25<\/td> | water\/glyc.\/oil<\/td> | HCX PL Fluotar L<\/td><\/tr> | 40x<\/td> | 1.25<\/td> | 0.10<\/td> | oil<\/td> | HCX PL APO CS<\/td><\/tr> | 63x<\/td> | 1.40<\/td> | 0.14<\/td> | oil<\/td> | HCX PL APO CS<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n | Detectors<\/h2>\n\n\n\n3x spectral PMT detector (Photo Multiplier Tube) Extra info<\/h2>\n\n\n\nLeica LAS-AF software with: Motorized stage with inserts for slides, 35mm dishes, and multiwell plates Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions Leica SP5 laser scanning confocal with AOBS Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 20x 0.70 0.25 water\/glyc.\/oil HCX PL Fluotar L 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1425","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP5 laser scanning confocal with AOBS Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 20x 0.70 0.25 water\/glyc.\/oil HCX PL Fluotar L 40x 1.25 0.10 oil HCX PL APO CS 63x 1.40 0.14 oil HCX PL APO CS…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1425"}],"version-history":[{"count":7,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425\/revisions"}],"predecessor-version":[{"id":3108,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1425\/revisions\/3108"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}} |