{"id":1427,"date":"2020-02-17T15:46:40","date_gmt":"2020-02-17T15:46:40","guid":{"rendered":"https:\/\/erasmusoic.nl\/2020\/?page_id=1427"},"modified":"2024-10-31T08:53:16","modified_gmt":"2024-10-31T08:53:16","slug":"leica-sp5-604-ee-604","status":"publish","type":"page","link":"https:\/\/erasmusoic.nl\/2020\/facility\/equipment\/leica-sp5-604-ee-604\/","title":{"rendered":"Leica SP5 604 \/ Ee-604"},"content":{"rendered":"\n<p class=\"has-text-align-center has-large-font-size wp-block-paragraph\">Leica SP5 confocal with AOBS, GalvoZ stage and Adaptive Focus Control (AFC)<\/p>\n\n\n<div class=\"wp-block-image is-style-circle-mask\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1024\" src=\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-1024x1024.jpg\" alt=\"\" class=\"wp-image-1354\" srcset=\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-1024x1024.jpg 1024w, https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-300x300.jpg 300w, https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-150x150.jpg 150w, https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-768x768.jpg 768w, https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604.jpg 1052w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n<h2 class=\"wp-block-heading\">Lasers<\/h2>\n\n\n\n<figure class=\"wp-block-image size-large is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"219\" src=\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-spectrum-1024x219.png\" alt=\"\" class=\"wp-image-1353\" srcset=\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-spectrum-1024x219.png 1024w, https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-spectrum-300x64.png 300w, https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-spectrum-768x165.png 768w, https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-spectrum-1200x257.png 1200w, https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/SP5-Ee604-spectrum.png 1400w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Objectives<\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>Magnification<\/strong><\/td><td><strong>Numerical<br>Aperture<\/strong><\/td><td><strong>Working<br>Distance (mm)<\/strong><\/td><td><strong>Immersion<\/strong><\/td><td><strong>Type<\/strong><\/td><\/tr><tr><td>5x<\/td><td>0.15<\/td><td>13.7<\/td><td>dry<\/td><td>HCX PL Fluotar<\/td><\/tr><tr><td>10x<\/td><td>0.40<\/td><td>2.2<\/td><td>dry<\/td><td>HCX PL APO CS<\/td><\/tr><tr><td>20x<\/td><td>0.70<\/td><td>0.59<\/td><td>dry<\/td><td>HCX PL APO CS<\/td><\/tr><tr><td>20x<\/td><td>0.70<\/td><td>0.25<\/td><td>water\/glyc.\/oil<\/td><td>HCX PL APO CS<\/td><\/tr><tr><td>40x<\/td><td>1.25<\/td><td>0.10<\/td><td>oil<\/td><td>HCX PL APO CS<\/td><\/tr><tr><td>63x<\/td><td>1.40<\/td><td>0.14<\/td><td>oil<\/td><td>HCX PL APO CS<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Detectors<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">2x spectral PMT (Photo Multiplier Tube) <br>1x spectral HyD (Hybrid detector)<br>1x transmission PMT for brightfield, DIC and polarization<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Extra info<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Leica LAS-AF software with:<br>Matrix screening software for high content screening<br>FRAP &amp; FRET modules<br>Live data mode<br><br>Leica DMi6000 inverted microscope stand with:<br>Adaptive Focus Control<br>GalvoZ stage with inserts for slides, 35mm dishes, 10cm dishes, and multi-well plates <br>Large incubator with heating and CO<sub>2<\/sub><br>Metal-Halide lamp for EPI fluorescence<br><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Applications<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Live cell confocal imaging under 5% CO<sub>2<\/sub> and 37<sup>o<\/sup>C conditions, also for multi-well plates.<br>FRAP, FRET, (advanced multi well)<br>Time lapse imaging<br>Fixed cell imaging<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Leica SP5 confocal with AOBS, GalvoZ stage and Adaptive Focus Control (AFC) Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 5x 0.15 13.7 dry HCX PL Fluotar 10x 0.40 2.2 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 20x 0.70 0.25 water\/glyc.\/oil HCX PL APO CS 40x 1.25 0.10 oil [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1427","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP5 confocal with AOBS, GalvoZ stage and Adaptive Focus Control (AFC) Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 5x 0.15 13.7 dry HCX PL Fluotar 10x 0.40 2.2 dry HCX PL APO CS 20x 0.70 0.59 dry HCX PL APO CS 20x 0.70 0.25 water\/glyc.\/oil HCX PL APO CS 40x 1.25 0.10 oil&hellip;","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1427"}],"version-history":[{"count":10,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1427\/revisions"}],"predecessor-version":[{"id":4522,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1427\/revisions\/4522"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}