{"id":1429,"date":"2020-02-17T16:04:24","date_gmt":"2020-02-17T16:04:24","guid":{"rendered":"https:\/\/erasmusoic.nl\/2020\/?page_id=1429"},"modified":"2024-01-04T07:24:50","modified_gmt":"2024-01-04T07:24:50","slug":"leica-sp5-intravital-ee-880","status":"publish","type":"page","link":"https:\/\/erasmusoic.nl\/2020\/facility\/equipment\/leica-sp5-intravital-ee-880\/","title":{"rendered":"Leica SP5 Intravital \/ Ee-880"},"content":{"rendered":"\n

Leica SP5 AOBS with multiphoton laser and OPO<\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

Lasers<\/h2>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Objectives<\/h2>\n\n\n\n
Magnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>		Working
Distance (mm)<\/strong><\/td>		Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>
1.6x<\/td>0.05<\/td>3.40<\/td>dry<\/td>HCX PL S-APO<\/td><\/tr>
10x<\/td>0.40<\/td>2.20<\/td>dry<\/td>HCX PL APO CS<\/td><\/tr>
20x<\/td>0.50<\/td>1.15<\/td>dry<\/td>HCX PL S-APO<\/td><\/tr>
20x<\/td>0.70<\/td>0.59<\/td>dry<\/td>HCX PL APO CS<\/td><\/tr>
20x<\/td>1.00<\/td>1.95<\/td>water<\/td>APO (dipping)<\/td><\/tr>
40x<\/td>1.25<\/td>0.10<\/td>oil<\/td>HCX PL APO CS<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Detectors<\/h2>\n\n\n\n

3x spectral PMT detector (Photo Multiplier Tube)
4x NDD (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization<\/p>\n\n\n\n

Filters<\/h2>\n\n\n\n
Non Descanned Detection (NDD) Port<\/strong><\/td>Emission<\/strong><\/td><\/tr>
NDD 1<\/td>430-450nm<\/td><\/tr>
NDD 2<\/td>465-500nm<\/td><\/tr>
NDD 3 <\/td>520-550nm<\/td><\/tr>
NDD 4<\/td>565-605nm<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Extra info<\/h2>\n\n\n\n

Multiphoton laser Coherent Chameleon Ultra (710-1040nm)
Multiphoton laser APE OPO (1100-1300nm)<\/p>\n\n\n\n

Leica LAS-AF software with:
Matrix screening software for high content screening<\/p>\n\n\n\n

DMi6000 upright microscope stand
Metal-Halide lamp for EPI fluorescence<\/p>\n\n\n\n

Applications<\/h2>\n\n\n\n

This confocal is especially equipped for intra vital imaging
(advanced multi well)
Time lapse imaging<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Leica SP5 AOBS with multiphoton laser and OPO Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 1.6x 0.05 3.40 dry HCX PL S-APO 10x 0.40 2.20 dry HCX PL APO CS 20x 0.50 1.15 dry HCX PL S-APO 20x 0.70 0.59 dry HCX PL APO CS 20x 1.00 1.95 water APO (dipping) 40x 1.25 0.10 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-1429","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Rob de Bree","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/robdebree\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP5 AOBS with multiphoton laser and OPO Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 1.6x 0.05 3.40 dry HCX PL S-APO 10x 0.40 2.20 dry HCX PL APO CS 20x 0.50 1.15 dry HCX PL S-APO 20x 0.70 0.59 dry HCX PL APO CS 20x 1.00 1.95 water APO (dipping) 40x 1.25 0.10…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1429","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=1429"}],"version-history":[{"count":7,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1429\/revisions"}],"predecessor-version":[{"id":3888,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/1429\/revisions\/3888"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=1429"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}