![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/08\/DSC_6459-edited-scaled.jpg\")
<\/figure>\n<\/div>\n\n\nLasers<\/h2>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/11\/SP8DLS_1315_spectrum-1024x180.png\")
<\/figure>\n\n\n\nConfocal Objectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.40<\/td> 2.20<\/td> dry<\/td> HC PL APO CS2<\/td><\/tr> 20x<\/td> 0.75<\/td> 0.62<\/td> dry<\/td> HC PL APO CS2<\/td><\/tr> 40x<\/td> 1.30<\/td> 0.24<\/td> oil<\/td> HC PL APO CS2<\/td><\/tr> 63x<\/td> 1.40<\/td> 0.14<\/td> oil<\/td> HC PL APO CS2<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nLight Sheet Objectives<\/h2>\n\n\n\nDLS illumination<\/strong><\/td><\/td> <\/td> <\/td> <\/td><\/tr> Magnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working distance<\/strong> (mm)<\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>1.6x<\/td> 0.05<\/td> 8.1<\/td> Dry<\/td> HC PL FLUORTAR L<\/td><\/tr> 2.5x<\/td> 0.07<\/td> 9.4<\/td> Dry<\/td> HC PL FLUORTAR<\/td><\/tr> 4x<\/td> 0.13<\/td> 17<\/td> Dry<\/td> HC PL FLUORTAR<\/td><\/tr> 5x<\/td> 0.15<\/td> 13.7<\/td> Dry<\/td> HC PL FLUORTAR<\/td><\/tr> <\/td> <\/td> <\/td> <\/td> <\/td><\/tr> DLS detection<\/strong><\/td><\/td> <\/td> <\/td> <\/td><\/tr> Magnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working distance<\/strong> (mm)<\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>5x<\/td> 0.15<\/td> 4.8<\/td> Water, BABB<\/td> HC PL FLUORTAR<\/td><\/tr> 10x<\/td> 0.30<\/td> 3.6<\/td> Water<\/td> HC APO L<\/td><\/tr> 20x<\/td> 0.50<\/td> 3.5<\/td> Water<\/td> HC APO L<\/td><\/tr> <\/td> <\/td> <\/td> <\/td> <\/td><\/tr> DLS mirror (TwinFlect)<\/strong><\/td>DLS illumination
objective<\/strong><\/td>DLS detection
objective<\/strong><\/td>Immersion<\/strong><\/td><\/td><\/tr> 2.5mm<\/td> 4x<\/td> 20x<\/td> Water<\/td> <\/td><\/tr> 5mm<\/td> 2.5x<\/td> 10x (or 20x)<\/td> Water<\/td> <\/td><\/tr> 7.8mm<\/td> 1.6x<\/td> 5x ( or 10x)<\/td> Water<\/td> <\/td><\/tr> 7.8mm<\/td> 1.6x<\/td> 5x<\/td> BABB<\/td> <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
2x spectral PMT (Photo Multiplier Tube)
2x spectral HyD (Hybrid detector)
1x transmission PMT
1x scCMOS camera (Leica DFC9000 GTC DLS, 2048 x 2048 pixels)<\/p>\n\n\n\n
<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
DLS emission band pass filter suitable for fluorophores excited with: 405nm, 488nm, 561nm and 633nm<\/p>\n\n\n\n
Leica LAS-X software with:
Navigator package
LAS-X 3D viewer
LAS-X Live data mode<\/p>\n\n\n\n
DMi8 inverted microscope stand with:
Automated motorized XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence.<\/p>\n\n\n\n
Okolab stage top incubator for Z-Galvo stage with feedback sensor,
humidified 5% CO2<\/sub> and objective heating.
Temperature data logging possible via LAS-X.
Okolab incubator inserts for 35mm & slide sized containers.<\/p>\n\n\n\n<\/p>\n\n\n\n
Applications<\/h2>\n\n\n\n
The DLS light sheet module from Leica (Digital Light Sheet), can be used to image specimen in the order from organoids to zebrafish embryo’s.
Live cell confocal imaging under CO2 and 37o<\/sup>C conditions
Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews
3D module for 3D rendering<\/p>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"Leica SP8 AOBS with Digital LightSheet (DLS), DMi8 microscope stand with Galvo Z stage and Adaptive Focus Control Lasers Confocal Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HC PL APO CS2 20x 0.75 0.62 dry HC PL APO CS2 40x 1.30 0.24 oil HC PL APO CS2 63x 1.40 0.14 oil […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2576","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Gert van Cappelen","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/gert-van-cappellen\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP8 AOBS with Digital LightSheet (DLS), DMi8 microscope stand with Galvo Z stage and Adaptive Focus Control Lasers Confocal Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HC PL APO CS2 20x 0.75 0.62 dry HC PL APO CS2 40x 1.30 0.24 oil HC PL APO CS2 63x 1.40 0.14 oil…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2576","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2576"}],"version-history":[{"count":25,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2576\/revisions"}],"predecessor-version":[{"id":3606,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2576\/revisions\/3606"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2576"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>\n\n\n\nConfocal Objectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.40<\/td> 2.20<\/td> dry<\/td> HC PL APO CS2<\/td><\/tr> 20x<\/td> 0.75<\/td> 0.62<\/td> dry<\/td> HC PL APO CS2<\/td><\/tr> 40x<\/td> 1.30<\/td> 0.24<\/td> oil<\/td> HC PL APO CS2<\/td><\/tr> 63x<\/td> 1.40<\/td> 0.14<\/td> oil<\/td> HC PL APO CS2<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nLight Sheet Objectives<\/h2>\n\n\n\nDLS illumination<\/strong><\/td><\/td> <\/td> <\/td> <\/td><\/tr> Magnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working distance<\/strong> (mm)<\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>1.6x<\/td> 0.05<\/td> 8.1<\/td> Dry<\/td> HC PL FLUORTAR L<\/td><\/tr> 2.5x<\/td> 0.07<\/td> 9.4<\/td> Dry<\/td> HC PL FLUORTAR<\/td><\/tr> 4x<\/td> 0.13<\/td> 17<\/td> Dry<\/td> HC PL FLUORTAR<\/td><\/tr> 5x<\/td> 0.15<\/td> 13.7<\/td> Dry<\/td> HC PL FLUORTAR<\/td><\/tr> <\/td> <\/td> <\/td> <\/td> <\/td><\/tr> DLS detection<\/strong><\/td><\/td> <\/td> <\/td> <\/td><\/tr> Magnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working distance<\/strong> (mm)<\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>5x<\/td> 0.15<\/td> 4.8<\/td> Water, BABB<\/td> HC PL FLUORTAR<\/td><\/tr> 10x<\/td> 0.30<\/td> 3.6<\/td> Water<\/td> HC APO L<\/td><\/tr> 20x<\/td> 0.50<\/td> 3.5<\/td> Water<\/td> HC APO L<\/td><\/tr> <\/td> <\/td> <\/td> <\/td> <\/td><\/tr> DLS mirror (TwinFlect)<\/strong><\/td>DLS illumination
objective<\/strong><\/td>DLS detection
objective<\/strong><\/td>Immersion<\/strong><\/td><\/td><\/tr> 2.5mm<\/td> 4x<\/td> 20x<\/td> Water<\/td> <\/td><\/tr> 5mm<\/td> 2.5x<\/td> 10x (or 20x)<\/td> Water<\/td> <\/td><\/tr> 7.8mm<\/td> 1.6x<\/td> 5x ( or 10x)<\/td> Water<\/td> <\/td><\/tr> 7.8mm<\/td> 1.6x<\/td> 5x<\/td> BABB<\/td> <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
2x spectral PMT (Photo Multiplier Tube)
2x spectral HyD (Hybrid detector)
1x transmission PMT
1x scCMOS camera (Leica DFC9000 GTC DLS, 2048 x 2048 pixels)<\/p>\n\n\n\n
<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
DLS emission band pass filter suitable for fluorophores excited with: 405nm, 488nm, 561nm and 633nm<\/p>\n\n\n\n
Leica LAS-X software with:
Navigator package
LAS-X 3D viewer
LAS-X Live data mode<\/p>\n\n\n\n
DMi8 inverted microscope stand with:
Automated motorized XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence.<\/p>\n\n\n\n
Okolab stage top incubator for Z-Galvo stage with feedback sensor,
humidified 5% CO2<\/sub> and objective heating.
Temperature data logging possible via LAS-X.
Okolab incubator inserts for 35mm & slide sized containers.<\/p>\n\n\n\n<\/p>\n\n\n\n
Applications<\/h2>\n\n\n\n
The DLS light sheet module from Leica (Digital Light Sheet), can be used to image specimen in the order from organoids to zebrafish embryo’s.
Live cell confocal imaging under CO2 and 37o<\/sup>C conditions
Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews
3D module for 3D rendering<\/p>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"Leica SP8 AOBS with Digital LightSheet (DLS), DMi8 microscope stand with Galvo Z stage and Adaptive Focus Control Lasers Confocal Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HC PL APO CS2 20x 0.75 0.62 dry HC PL APO CS2 40x 1.30 0.24 oil HC PL APO CS2 63x 1.40 0.14 oil […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2576","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Gert van Cappelen","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/gert-van-cappellen\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP8 AOBS with Digital LightSheet (DLS), DMi8 microscope stand with Galvo Z stage and Adaptive Focus Control Lasers Confocal Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 dry HC PL APO CS2 20x 0.75 0.62 dry HC PL APO CS2 40x 1.30 0.24 oil HC PL APO CS2 63x 1.40 0.14 oil…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2576","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2576"}],"version-history":[{"count":25,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2576\/revisions"}],"predecessor-version":[{"id":3606,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2576\/revisions\/3606"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2576"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Magnification<\/strong><\/td>| Numerical | Aperture<\/strong><\/td> Working | Distance (mm)<\/strong><\/td> Immersion<\/strong><\/td> | Type<\/strong><\/td><\/tr> | 10x<\/td> | 0.40<\/td> | 2.20<\/td> | dry<\/td> | HC PL APO CS2<\/td><\/tr> | 20x<\/td> | 0.75<\/td> | 0.62<\/td> | dry<\/td> | HC PL APO CS2<\/td><\/tr> | 40x<\/td> | 1.30<\/td> | 0.24<\/td> | oil<\/td> | HC PL APO CS2<\/td><\/tr> | 63x<\/td> | 1.40<\/td> | 0.14<\/td> | oil<\/td> | HC PL APO CS2<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n | Light Sheet Objectives<\/h2>\n\n\n\n
|