{"id":2606,"date":"2020-10-09T08:56:42","date_gmt":"2020-10-09T08:56:42","guid":{"rendered":"https:\/\/erasmusoic.nl\/?page_id=2606"},"modified":"2021-11-18T13:45:07","modified_gmt":"2021-11-18T13:45:07","slug":"leica-sp8-cil-ee-164","status":"publish","type":"page","link":"https:\/\/erasmusoic.nl\/2020\/facility\/equipment\/leica-sp8-cil-ee-164\/","title":{"rendered":"Leica SP8 CIL \/ Ee-164"},"content":{"rendered":"\n

Leica SP8 Low Incidence Angle optics, C1 lab, Galvo Z stage & Adaptive Focus Control<\/p>\n\n\n\n

\"\"<\/figure><\/div>\n\n\n\n

Lasers<\/h2>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Objectives<\/h2>\n\n\n\n
Magnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>		Working
Distance (mm)<\/strong><\/td>		Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>
10x<\/td>0.40<\/td>2.20<\/td>Air<\/td>HC PL APO CS2<\/td><\/tr>
20x<\/td>0.75<\/td>0.62<\/td>Air<\/td>HC PL APO CS2<\/td><\/tr>
40x<\/td>1.30<\/td>0.24<\/td>Oil<\/td>HC PL APO CS2<\/td><\/tr>
63x<\/td>1.40<\/td>0.14<\/td>Oil<\/td>HC PL APO CS2<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Detectors<\/h2>\n\n\n\n

2x spectral PMT (Photo Multiplier Tube)
1x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization<\/p>\n\n\n\n

Extra info<\/h2>\n\n\n\n

Leica LAS-X software with navigator package

DMi8 inverted microscope stand with:
Automated XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence<\/p>\n\n\n\n

Okolab cage incubator around microscope stand with:
Okolab incubator inserts for 35mm, slide sized containers & multi-well plates.
For 37\u00b0C conditions with humidified 5% CO2.
Temperature data logging possible via LAS-X.<\/p>\n\n\n\n

Applications<\/h2>\n\n\n\n

This microscope is situated in an C1 lab for confocal imaging experiments combined with radioactivity<\/p>\n\n\n\n

Live cell confocal imaging under CO2<\/sub> and 37o<\/sup>C conditions Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews<\/p>\n","protected":false},"excerpt":{"rendered":"

Leica SP8 Low Incidence Angle optics, C1 lab, Galvo Z stage & Adaptive Focus Control Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 Air HC PL APO CS2 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO CS2 […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2606","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Gert van Cappelen","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/gert-van-cappellen\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP8 Low Incidence Angle optics, C1 lab, Galvo Z stage & Adaptive Focus Control Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 Air HC PL APO CS2 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO CS2…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2606","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2606"}],"version-history":[{"count":13,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2606\/revisions"}],"predecessor-version":[{"id":3005,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2606\/revisions\/3005"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2606"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}