{"id":2618,"date":"2020-09-02T14:17:50","date_gmt":"2020-09-02T14:17:50","guid":{"rendered":"https:\/\/erasmusoic.nl\/?page_id=2618"},"modified":"2026-02-04T12:16:34","modified_gmt":"2026-02-04T12:16:34","slug":"leica-sp8-sted-be-311","status":"publish","type":"page","link":"https:\/\/erasmusoic.nl\/2020\/facility\/equipment\/leica-sp8-sted-be-311\/","title":{"rendered":"Leica SP8 STED \/ Be-311"},"content":{"rendered":"\n

Leica SP8 AOBS with gated 3D tauSTED, white light laser, lightning and FALCON<\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

Lasers<\/h2>\n\n\n\n
\"\"<\/figure>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Objectives<\/h2>\n\n\n\n
Magnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>		Working
Distance (mm)<\/strong><\/td>		Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>
20x<\/td>0.75<\/td>0.62<\/td>Air<\/td>HC PL APO CS2<\/td><\/tr>
40x<\/td>1.30<\/td>0.24<\/td>Oil<\/td>HC PL APO CS2<\/td><\/tr>
63x<\/td>1.40<\/td>0.14<\/td>Oil<\/td>HC PL APO CS2<\/td><\/tr>
100x<\/td>1.40<\/td>0.13<\/td>Oil<\/td>HC PL APO CS2 STED<\/td><\/tr>
86x<\/td>1.20<\/td>0.3<\/td>Water<\/td>HC PL APO CS2 STED<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Detectors<\/h2>\n\n\n\n

2x spectral PMT (Photo Multiplier Tube)
2x gated spectral GaAsP HyD (Hybrid detector for gated imaging)
1x transmission PMT for brightfield, DIC and polarization<\/p>\n\n\n\n

Extra info<\/h2>\n\n\n\n

3x STED depletion lasers: 592, 660 & 775nm<\/p>\n\n\n\n

Leica LAS-X software with:
Navigator package
LAX-X 3D viewer
Leica Lightning deconvolution<\/p>\n\n\n\n

DMi8 inverted microscope stand with:
Automated motorized XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence.<\/p>\n\n\n\n

Tokai Hit stage top incubator for Z-Galvo stage with
humidified 5% CO2<\/sub> and objective heating.
Stage top incubator inserts for 35mm & slide sized containers.<\/p>\n\n\n\n

Applications<\/h2>\n\n\n\n

Super resolution imaging with (3D) STED, combined with lifetime; Tau-STED
Confocal imaging combined with lifetime imaging; FALCON
Confocal imaging combined with deconvolution; Lightning
Spectral freedom with White Light Laser 470-670nm range
Navigator module for fast overviews<\/p>\n","protected":false},"excerpt":{"rendered":"

Leica SP8 AOBS with gated 3D tauSTED, white light laser, lightning and FALCON Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO CS2 100x 1.40 0.13 Oil HC PL APO CS2 STED 86x […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2618","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Gert van Cappelen","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/gert-van-cappellen\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP8 AOBS with gated 3D tauSTED, white light laser, lightning and FALCON Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO CS2 100x 1.40 0.13 Oil HC PL APO CS2 STED 86x…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2618","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2618"}],"version-history":[{"count":10,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2618\/revisions"}],"predecessor-version":[{"id":5069,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2618\/revisions\/5069"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2618"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}