![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/09\/DSC_6458-edited-1024x1024.jpg\")
<\/figure><\/div>\n\n\n\nLasers<\/h2>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/11\/SP8_775a_spectrum-1024x180.png\")
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.40<\/td> 2.20<\/td> Air<\/td> HC PL APO CS2<\/td><\/tr> 20x<\/td> 0.75<\/td> 0.62<\/td> Air<\/td> HC PL APO CS2<\/td><\/tr> 40x<\/td> 1.30<\/td> 0.24<\/td> Oil<\/td> HC PL APO CS2<\/td><\/tr> 63x<\/td> 1.40<\/td> 0.14<\/td> Oil<\/td> HC PL APO CS2<\/td><\/tr> 40x<\/td> 0.60<\/td> 0.36<\/td> Glycerol<\/td> Zeiss Ultrafluar<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
2x spectral PMT (Photo Multiplier Tube)
2x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
Deep UV (266nm & 355nm) system by Rapp OptoElectronics for DNA damage optogenetics by laser scanning unit<\/p>\n\n\n\n
Leica LAS-X software with:
Navigator package
LAS-X Live data mode
FRAP module
Leica Lightning deconvolution<\/p>\n\n\n\n
DMi8 inverted microscope stand with:
Automated XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence<\/p>\n\n\n\n
Okolab stage top incubator for Z-Galvo stage with feedback sensor,
humidified 5% CO2<\/sub> and objective heating.
Temperature data logging possible via LAS-X.
Okolab incubator inserts for 35mm & slide sized containers.<\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
This microscope is especially equipped for making localized DNA damage
Live cell confocal imaging under CO2 and 37\u00b0C conditions
FRAP, FRET, (advanced multi location) time lapse imaging
Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews<\/p>\n","protected":false},"excerpt":{"rendered":"
Leica SP8 AOBS, UV laser by Rapp OptoElectronics, Galvo Z stage & Adaptive Focus Control (AFC) Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 Air HC PL APO CS2 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2632","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Gert van Cappelen","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/gert-van-cappellen\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP8 AOBS, UV laser by Rapp OptoElectronics, Galvo Z stage & Adaptive Focus Control (AFC) Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 Air HC PL APO CS2 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2632"}],"version-history":[{"count":11,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632\/revisions"}],"predecessor-version":[{"id":2991,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632\/revisions\/2991"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2632"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.40<\/td> 2.20<\/td> Air<\/td> HC PL APO CS2<\/td><\/tr> 20x<\/td> 0.75<\/td> 0.62<\/td> Air<\/td> HC PL APO CS2<\/td><\/tr> 40x<\/td> 1.30<\/td> 0.24<\/td> Oil<\/td> HC PL APO CS2<\/td><\/tr> 63x<\/td> 1.40<\/td> 0.14<\/td> Oil<\/td> HC PL APO CS2<\/td><\/tr> 40x<\/td> 0.60<\/td> 0.36<\/td> Glycerol<\/td> Zeiss Ultrafluar<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nDetectors<\/h2>\n\n\n\n
2x spectral PMT (Photo Multiplier Tube)
2x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
Deep UV (266nm & 355nm) system by Rapp OptoElectronics for DNA damage optogenetics by laser scanning unit<\/p>\n\n\n\n
Leica LAS-X software with:
Navigator package
LAS-X Live data mode
FRAP module
Leica Lightning deconvolution<\/p>\n\n\n\n
DMi8 inverted microscope stand with:
Automated XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence<\/p>\n\n\n\n
Okolab stage top incubator for Z-Galvo stage with feedback sensor,
humidified 5% CO2<\/sub> and objective heating.
Temperature data logging possible via LAS-X.
Okolab incubator inserts for 35mm & slide sized containers.<\/p>\n\n\n\nApplications<\/h2>\n\n\n\n
This microscope is especially equipped for making localized DNA damage
Live cell confocal imaging under CO2 and 37\u00b0C conditions
FRAP, FRET, (advanced multi location) time lapse imaging
Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews<\/p>\n","protected":false},"excerpt":{"rendered":"
Leica SP8 AOBS, UV laser by Rapp OptoElectronics, Galvo Z stage & Adaptive Focus Control (AFC) Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 Air HC PL APO CS2 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2632","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Gert van Cappelen","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/gert-van-cappellen\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP8 AOBS, UV laser by Rapp OptoElectronics, Galvo Z stage & Adaptive Focus Control (AFC) Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 Air HC PL APO CS2 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2632"}],"version-history":[{"count":11,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632\/revisions"}],"predecessor-version":[{"id":2991,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632\/revisions\/2991"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2632"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Magnification<\/strong><\/td>| Numerical | Aperture<\/strong><\/td> Working | Distance (mm)<\/strong><\/td> Immersion<\/strong><\/td> | Type<\/strong><\/td><\/tr> | 10x<\/td> | 0.40<\/td> | 2.20<\/td> | Air<\/td> | HC PL APO CS2<\/td><\/tr> | 20x<\/td> | 0.75<\/td> | 0.62<\/td> | Air<\/td> | HC PL APO CS2<\/td><\/tr> | 40x<\/td> | 1.30<\/td> | 0.24<\/td> | Oil<\/td> | HC PL APO CS2<\/td><\/tr> | 63x<\/td> | 1.40<\/td> | 0.14<\/td> | Oil<\/td> | HC PL APO CS2<\/td><\/tr> | 40x<\/td> | 0.60<\/td> | 0.36<\/td> | Glycerol<\/td> | Zeiss Ultrafluar<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n | Detectors<\/h2>\n\n\n\n2x spectral PMT (Photo Multiplier Tube) Extra info<\/h2>\n\n\n\nDeep UV (266nm & 355nm) system by Rapp OptoElectronics for DNA damage optogenetics by laser scanning unit<\/p>\n\n\n\n Leica LAS-X software with: DMi8 inverted microscope stand with: Okolab stage top incubator for Z-Galvo stage with feedback sensor, This microscope is especially equipped for making localized DNA damage Leica SP8 AOBS, UV laser by Rapp OptoElectronics, Galvo Z stage & Adaptive Focus Control (AFC) Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 Air HC PL APO CS2 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2632","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Gert van Cappelen","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/gert-van-cappellen\/"},"uagb_comment_info":0,"uagb_excerpt":"Leica SP8 AOBS, UV laser by Rapp OptoElectronics, Galvo Z stage & Adaptive Focus Control (AFC) Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.40 2.20 Air HC PL APO CS2 20x 0.75 0.62 Air HC PL APO CS2 40x 1.30 0.24 Oil HC PL APO CS2 63x 1.40 0.14 Oil HC PL APO…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2632"}],"version-history":[{"count":11,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632\/revisions"}],"predecessor-version":[{"id":2991,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2632\/revisions\/2991"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2632"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}} |