![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/11\/RCM-bypass-transparent-400x288-2.png\"){kind=spacer}
<\/figure>\n<\/div>\n\n\nLasers<\/h2>\n\n\n\n![\"\"](\"https:\/\/erasmusoic.nl\/2020\/wp-content\/uploads\/2020\/02\/Nikon-TIRF-spectrum-1024x219.png\")
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical<\/strong>
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.45<\/td> 4.00<\/td> dry<\/td> Plan APO DIC<\/td><\/tr> 60x<\/td> 1.45<\/td> 0.13<\/td> oil<\/td> Plan APO TIRF<\/td><\/tr> 100x<\/td> 1.49<\/td> 0.12<\/td> oil<\/td> Plan APO TIRF<\/td><\/tr> 100x<\/td> 1.40<\/td> 0.13<\/td> oil<\/td> Plan APO VC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nPixel size<\/h2>\n\n\n\nObjective<\/strong><\/td>Sweep factor 1<\/strong><\/td>Sweep factor 2<\/strong><\/td><\/tr>60x NA 1.45 <\/td> 144 nm<\/td> 72 nm<\/td><\/tr> 100x NA1.49 <\/td> 84 nm<\/td> 42 nm<\/td><\/tr><\/tbody><\/table>Pixel size in XY, with a field of view of 768 x 768 pixels per channel <\/figcaption><\/figure>\n\n\n\n<\/p>\n\n\n\n\n\n
Camera<\/h2>\n\n\n\n
Hamamatsu Orca Flash 4 scCMOS camera 2048×2048 pixels
Field of view per channel is 768 x 768 pixels<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
Imaging under 5% CO2 and 30\/37\u00b0C conditions, with stage top incubator, CO2 controller and objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
Hardware autofocus: Perfect Focus System (Nikon)
RCM unit (Confocal.nl) with \u201cChurchwindow\u201d emmision filter Blue\/Green\/Red\/FarRed<\/p>\n\n\n\n
<\/p>\n\n\n\n
Applications<\/h2>\n\n\n\n
ReScanConfocal Microscopy: for a higher resolution image with the optical sectioning of a confocal
Live cell confocal imaging under CO2<\/sub> and 37\u00b0C conditions
Fixed cell imaging<\/p>\n\n\n\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Rescan confocal unit by confocal.nl with Nikon Ti inverted stand Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.45 4.00 dry Plan APO DIC 60x 1.45 0.13 oil Plan APO TIRF 100x 1.49 0.12 oil Plan APO TIRF 100x 1.40 0.13 oil Plan APO VC Pixel size Objective Sweep factor 1 Sweep factor 2 […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2777","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Martijn de Gruiter","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/martijn-de-gruiter\/"},"uagb_comment_info":0,"uagb_excerpt":"Rescan confocal unit by confocal.nl with Nikon Ti inverted stand Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.45 4.00 dry Plan APO DIC 60x 1.45 0.13 oil Plan APO TIRF 100x 1.49 0.12 oil Plan APO TIRF 100x 1.40 0.13 oil Plan APO VC Pixel size Objective Sweep factor 1 Sweep factor 2…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2777","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2777"}],"version-history":[{"count":6,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2777\/revisions"}],"predecessor-version":[{"id":4278,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2777\/revisions\/4278"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2777"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>\n\n\n\nObjectives<\/h2>\n\n\n\nMagnification<\/strong><\/td>Numerical<\/strong>
Aperture<\/strong><\/td>Working
Distance (mm)<\/strong><\/td>Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>10x<\/td> 0.45<\/td> 4.00<\/td> dry<\/td> Plan APO DIC<\/td><\/tr> 60x<\/td> 1.45<\/td> 0.13<\/td> oil<\/td> Plan APO TIRF<\/td><\/tr> 100x<\/td> 1.49<\/td> 0.12<\/td> oil<\/td> Plan APO TIRF<\/td><\/tr> 100x<\/td> 1.40<\/td> 0.13<\/td> oil<\/td> Plan APO VC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\nPixel size<\/h2>\n\n\n\nObjective<\/strong><\/td>Sweep factor 1<\/strong><\/td>Sweep factor 2<\/strong><\/td><\/tr>60x NA 1.45 <\/td> 144 nm<\/td> 72 nm<\/td><\/tr> 100x NA1.49 <\/td> 84 nm<\/td> 42 nm<\/td><\/tr><\/tbody><\/table>Pixel size in XY, with a field of view of 768 x 768 pixels per channel <\/figcaption><\/figure>\n\n\n\n<\/p>\n\n\n\n\n\n
Camera<\/h2>\n\n\n\n
Hamamatsu Orca Flash 4 scCMOS camera 2048×2048 pixels
Field of view per channel is 768 x 768 pixels<\/p>\n\n\n\n
Extra info<\/h2>\n\n\n\n
Imaging under 5% CO2 and 30\/37\u00b0C conditions, with stage top incubator, CO2 controller and objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
Hardware autofocus: Perfect Focus System (Nikon)
RCM unit (Confocal.nl) with \u201cChurchwindow\u201d emmision filter Blue\/Green\/Red\/FarRed<\/p>\n\n\n\n
<\/p>\n\n\n\n
Applications<\/h2>\n\n\n\n
ReScanConfocal Microscopy: for a higher resolution image with the optical sectioning of a confocal
Live cell confocal imaging under CO2<\/sub> and 37\u00b0C conditions
Fixed cell imaging<\/p>\n\n\n\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Rescan confocal unit by confocal.nl with Nikon Ti inverted stand Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.45 4.00 dry Plan APO DIC 60x 1.45 0.13 oil Plan APO TIRF 100x 1.49 0.12 oil Plan APO TIRF 100x 1.40 0.13 oil Plan APO VC Pixel size Objective Sweep factor 1 Sweep factor 2 […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-2777","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Martijn de Gruiter","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/martijn-de-gruiter\/"},"uagb_comment_info":0,"uagb_excerpt":"Rescan confocal unit by confocal.nl with Nikon Ti inverted stand Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 10x 0.45 4.00 dry Plan APO DIC 60x 1.45 0.13 oil Plan APO TIRF 100x 1.49 0.12 oil Plan APO TIRF 100x 1.40 0.13 oil Plan APO VC Pixel size Objective Sweep factor 1 Sweep factor 2…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2777","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=2777"}],"version-history":[{"count":6,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2777\/revisions"}],"predecessor-version":[{"id":4278,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/2777\/revisions\/4278"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=2777"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Magnification<\/strong><\/td>| Numerical<\/strong> | Aperture<\/strong><\/td> Working | Distance (mm)<\/strong><\/td> Immersion<\/strong><\/td> | Type<\/strong><\/td><\/tr> | 10x<\/td> | 0.45<\/td> | 4.00<\/td> | dry<\/td> | Plan APO DIC<\/td><\/tr> | 60x<\/td> | 1.45<\/td> | 0.13<\/td> | oil<\/td> | Plan APO TIRF<\/td><\/tr> | 100x<\/td> | 1.49<\/td> | 0.12<\/td> | oil<\/td> | Plan APO TIRF<\/td><\/tr> | 100x<\/td> | 1.40<\/td> | 0.13<\/td> | oil<\/td> | Plan APO VC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n | Pixel size<\/h2>\n\n\n\n
|