{"id":3071,"date":"2022-01-17T11:02:16","date_gmt":"2022-01-17T11:02:16","guid":{"rendered":"https:\/\/erasmusoic.nl\/?page_id=3071"},"modified":"2022-01-17T14:02:09","modified_gmt":"2022-01-17T14:02:09","slug":"opera-phenix-hcs-system","status":"publish","type":"page","link":"https:\/\/erasmusoic.nl\/2020\/facility\/equipment\/opera-phenix-hcs-system\/","title":{"rendered":"Opera Phenix HCS system"},"content":{"rendered":"\n

High Content Screening System with automation and liquid handling, Perkin Elmer<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Lasers<\/h2>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Objectives<\/h2>\n\n\n\n
Magnification<\/strong><\/td>Numerical<\/strong>
Aperture<\/strong><\/td>		Working
Distance (mm)<\/strong><\/td>		Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>
5x <\/td>0.16<\/td> long WD <\/td>Air<\/td><\/td><\/tr>
10x<\/td>0.3<\/td> long WD<\/td>Air<\/td><\/td><\/tr>
20x <\/td>0.4<\/td>8.28<\/td>Air<\/td><\/td><\/tr>
40x<\/td>1.1<\/td><\/td>Water<\/td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Camera<\/h2>\n\n\n\n

2x sCMOS camera (4.4 megapixels, 6.5 \u03bcm x 6.5 \u03bcm pixel size)<\/p>\n\n\n\n

Sample Holders<\/h2>\n\n\n\n

Multi-well (96\/384) plates, up to 4 slides simultaneously
(Other sample types can be discussed)<\/p>\n\n\n\n

Automation and liquid handling<\/h2>\n\n\n\n

Robotics: Plate handler workstation SX 2
Incubator: 40 multi-well plate incubator (37\u00b0C, 5% CO2<\/sub>, 95% humidity)
Dispensor: Janus 4-tip
Washer: ELx405 (50 – 3000 \u03bcL\/well)
Barcode reader<\/p>\n\n\n\n

Extra info<\/h2>\n\n\n\n

System contains both Spinning disk confocal and Widefield fluorescence optics.
Spinning disk module with Confocal SynchronyTM<\/sup> Optics (Perkin Elmer).
Imaging modes: Fluorescence, brightfield and digital phase contrast
Available band pass filters: 435-480, 435-515, 435-550, 500-550, 570-630 and 650-760 nm<\/p>\n\n\n\n

Available analysis software <\/h2>\n\n\n\n

Harmony\u00ae High Content Imaging and Analysis Software provides a complete solution to set up assays and automate your high content imaging experiments, acquire images and analyze data, and then store, retrieve and present the results.<\/p>\n\n\n\n

The PhenoLOGIC\u2122 machine-learning option enables our High Content Analysis software to recognize different cell populations and regions using a simple learn-by-example approach. PhenoLOGIC sets parameters for optimal image segmentation and cell classification. The software combines the most meaningful parameters, whether it\u2019s two, three, four or more, to achieve accurate classification of cells. The result is highly robust and statistically relevant results.<\/p>\n\n\n\n

Columbus\u2122 Scope system is a universal high-volume image data storage and analysis system that rings access to images from a wide range of sources including all major high content screening instruments via the Internet.<\/p>\n\n\n\n

ImageJ\/Fiji is a public domain Java image processing and analysis package. Customized image analysis can be performed in collaboration with Erasmus Optical Imaging Centre (OIC) and Biomedical Imaging Group Rotterdam (BIGR).<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

High Content Screening System with automation and liquid handling, Perkin Elmer Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 5x 0.16 long WD Air 10x 0.3 long WD Air 20x 0.4 8.28 Air 40x 1.1 Water Camera 2x sCMOS camera (4.4 megapixels, 6.5 \u03bcm x 6.5 \u03bcm pixel size) Sample Holders Multi-well (96\/384) plates, up […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-3071","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Martijn de Gruiter","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/martijn-de-gruiter\/"},"uagb_comment_info":0,"uagb_excerpt":"High Content Screening System with automation and liquid handling, Perkin Elmer Lasers Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 5x 0.16 long WD Air 10x 0.3 long WD Air 20x 0.4 8.28 Air 40x 1.1 Water Camera 2x sCMOS camera (4.4 megapixels, 6.5 \u03bcm x 6.5 \u03bcm pixel size) Sample Holders Multi-well (96\/384) plates, up…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/3071","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=3071"}],"version-history":[{"count":5,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/3071\/revisions"}],"predecessor-version":[{"id":3082,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/3071\/revisions\/3082"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=3071"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}