{"id":4750,"date":"2025-09-05T14:56:49","date_gmt":"2025-09-05T14:56:49","guid":{"rendered":"https:\/\/erasmusoic.nl\/?page_id=4750"},"modified":"2025-11-06T11:08:19","modified_gmt":"2025-11-06T11:08:19","slug":"zeiss-8-slide-wide-field-be311","status":"publish","type":"page","link":"https:\/\/erasmusoic.nl\/2020\/facility\/equipment\/zeiss-8-slide-wide-field-be311\/","title":{"rendered":"Zeiss 8-slide Wide Field – Be311"},"content":{"rendered":"\n

Zeiss AxioImager II upright microscope<\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

Spectrum<\/h2>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Objectives<\/h2>\n\n\n\n
Magnification<\/strong><\/td>Numerical
Aperture<\/strong><\/td>		Working
Distance (mm)<\/strong><\/td>		Immersion<\/strong><\/td>Type<\/strong><\/td><\/tr>
2.5x<\/td>0.06<\/td>9.4<\/td>Air<\/td>A-Plan, Pol<\/td><\/tr>
5x<\/td>0.12<\/td>9.9<\/td>Air<\/td>A Plan, Pol<\/td><\/tr>
10x<\/td>0.25<\/td>6<\/td>Air<\/td>N Achroplan<\/td><\/tr>
20x<\/td>0.5<\/td>2.0<\/td>Air<\/td>EC Plan-NeoFluar, Pol<\/td><\/tr>
40x<\/td>0.47<\/td>0.47<\/td>Air<\/td>Plan-NeoFluar, Pol<\/td><\/tr>
63x<\/td>0.95<\/td>0.12<\/td>Air<\/td>EC Plan-Neofluar Korr., Pol<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Detectors<\/h2>\n\n\n\n

Axiocam MRm rev. 3 monochrome fluorescence camera
Axiocam 208 color camera for brightfield & polarisation acquisition<\/p>\n\n\n\n

Filters<\/h2>\n\n\n\n
Colour<\/strong><\/td>Exitation<\/strong><\/td>Emission<\/strong><\/td>Type<\/strong><\/td><\/tr>
Blue<\/td>BP 320-380<\/td>BP 420-470<\/td>DAPI, Hoechst<\/td><\/tr>
Cyan<\/td>BP 426-447<\/td>BP 468-492<\/td>Alexa Fluor 430, Aqua, DCC<\/td><\/tr>
Green<\/td>BP 477-500<\/td>BP 514-540<\/td>Alexa Fluor 488, FITC<\/td><\/tr>
Yellow<\/td>BP 523-548<\/td>BP 564-595<\/td>Alexa 532, Gold<\/td><\/tr>
Orange<\/td>BP 540-580<\/td>BP 575-640<\/td>Alexa Fluor 555, Cy3<\/td><\/tr>
Red<\/td>BP 573-596<\/td>BP 610-645<\/td>Alexa Fluor 594, TexasRed<\/td><\/tr>
Far Red <\/td>BP 625-655<\/td>BP655-715<\/td>Alexa Fluor 647, Cy5<\/td><\/tr>
Near-IR<\/td>BP 715-755<\/td>BP 765-855<\/td>Cy7<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Extra info<\/h2>\n\n\n\n

XY stage with capability of loading up to 8 slides and tile-scan capabilities
Z-drive for z-stacks
Colibri 7 LED lightsource
Zen Blue imaging software<\/p>\n\n\n\n

Applications<\/h2>\n\n\n\n

Widefield microscope with LED illumination system for automated and faster overview scans. Can be used before imaging samples on a confocal. The obtained overview can be used to locate area’s of interest to image with higher resolution and optical sectioning on a confocal microscope.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Zeiss AxioImager II upright microscope Spectrum Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 2.5x 0.06 9.4 Air A-Plan, Pol 5x 0.12 9.9 Air A Plan, Pol 10x 0.25 6 Air N Achroplan 20x 0.5 2.0 Air EC Plan-NeoFluar, Pol 40x 0.47 0.47 Air Plan-NeoFluar, Pol 63x 0.95 0.12 Air EC Plan-Neofluar Korr., Pol Detectors Axiocam […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-4750","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false,"twentytwenty-fullscreen":false},"uagb_author_info":{"display_name":"Martijn de Gruiter","author_link":"https:\/\/erasmusoic.nl\/2020\/author\/martijn-de-gruiter\/"},"uagb_comment_info":0,"uagb_excerpt":"Zeiss AxioImager II upright microscope Spectrum Objectives Magnification NumericalAperture WorkingDistance (mm) Immersion Type 2.5x 0.06 9.4 Air A-Plan, Pol 5x 0.12 9.9 Air A Plan, Pol 10x 0.25 6 Air N Achroplan 20x 0.5 2.0 Air EC Plan-NeoFluar, Pol 40x 0.47 0.47 Air Plan-NeoFluar, Pol 63x 0.95 0.12 Air EC Plan-Neofluar Korr., Pol Detectors Axiocam…","_links":{"self":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/4750","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/comments?post=4750"}],"version-history":[{"count":4,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/4750\/revisions"}],"predecessor-version":[{"id":4973,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/4750\/revisions\/4973"}],"up":[{"embeddable":true,"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/pages\/999"}],"wp:attachment":[{"href":"https:\/\/erasmusoic.nl\/2020\/wp-json\/wp\/v2\/media?parent=4750"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}