Axio Observer 7 microscope for BSL2 live cell imaging
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance | Immersion | Type |
| 2.5x | 0.085 | 8.8 | Dry | EC Plan-Neofluar |
| 5x | 0.16 | 18.5 | Dry | EC Plan-Neofluar |
| 10x | 0.3 | 2 | Dry | EC Plan-Apochromat |
| 20x | 0.8 | 0.55 | Dry | Plan-Apochromat |
| 40x | 1.3 | 0.21 | Oil | Plan-Apochromat DIC (UV)VIS-IR |
| 63x | 1.4 | 0.19 | Oil | Plan-Apochromat |
Detectors
2x PMT Spectral detection (spectral range: 405-700)
1x CH-GaAsP (spectral range: 405-700)
Transmitted light detector T-PMT 2
Filter
Filter set 90 HE LED
Excitation wavelengths: 385, 475, 555 and 630 nm.
Beam splitter QBS 405 + 493 + 575 + 653
Emission filter QBP 425/30 + 514/30 + 592/25 + 709/100
Extra information
Light Source Viluma 5 RGB-UV for EPI Fluorescence
MircoLED3 for brightfield imaging
Axiocam 305 monochrome Camera
Motorized scanning stage and focus drive
AI Sample Finder for quick tissue localisation
ZEN Blue software with Toolkits for:
Advanced SMART Live cell 2D/3D Acquisition
Ibidi™ Temperature and humidified CO2/O2 Controler with heated plate, glass lid and objective heater and Ibidi™ IncuControl software.
Inserts for: 35mm, 60mm dishes, (chamber) slides.
Applications
This microscope is especially equipped for BSL2 live cell confocal imaging under CO2 and 37°C conditions.