inverted lightsheet microscope, subcellular resolution in 3D cultured cells
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x Illumination objective | 0.3 | 3.5 | water | Nikon CFI Plan Fluor |
| 25x Detection objective | 1.1 | 2 | water | Nikon CFI Apo |
Camera
2x high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3 (2048 × 2048 pixels of 6.5 µm × 6.5 µm size)
Extra info
Imaging under 5% CO2 and 30/37°C conditions.
Cells must be cultured in InviSpim compatible container.
Maximum frame rate >80 fps at full frame, up to 500 fps at subframe cropping.
Available filters: BP 430-480, LP 498, BP 497-554, BP 520-550, LP 572, BP 580-627
Applications
Light Sheet microscope suited for subcellular resolution imaging in 3D cell cultures of stem cells and a small area of organoids.
Multiple lightsheet beams available for acquisition (Gaussian, Bessel, Lattice, Airy beam).