InVi SPIM / Ee-1078

inverted lightsheet microscope, subcellular resolution in 3D cultured cells

Lasers

Objectives

MagnificationNumerical
Aperture
Working
Distance (mm)
ImmersionType
10x
Illumination objective
0.33.5waterNikon CFI Plan Fluor
25x
Detection objective
1.12waterNikon CFI Apo

Camera

2x high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3 (2048 × 2048 pixels of 6.5 µm × 6.5 µm size)

Extra info

Imaging under 5% CO2 and 30/37°C conditions.
Cells must be cultured in InviSpim compatible container.
Maximum frame rate >80 fps at full frame, up to 500 fps at subframe cropping.
Available filters: BP 430-480, LP 498, BP 497-554, BP 520-550, LP 572, BP 580-627

Applications

Light Sheet microscope suited for subcellular resolution imaging in 3D cell cultures of stem cells and a small area of organoids.
Multiple lightsheet beams available for acquisition (Gaussian, Bessel, Lattice, Airy beam).