Leica SP8 DLS / Ee-1315

Leica SP8 AOBS with Digital LightSheet (DLS), DMi8 microscope stand with Galvo Z stage and Adaptive Focus Control

Lasers

Confocal Objectives

MagnificationNumerical
Aperture
Working
Distance (mm)
ImmersionType
10x0.402.20dryHC PL APO CS2
20x0.750.62dryHC PL APO CS2
40x1.300.24oilHC PL APO CS2
63x1.400.14oilHC PL APO CS2

Light Sheet Objectives

DLS illumination
MagnificationNumerical
Aperture
ImmersionType
1.6x0.05DryHC PL FLUORTAR L
2.5x0.07DryHC PL FLUORTAR
4x0.13DryHC PL FLUORTAR
5x0.50DryHC PL FLUORTAR
DLS detection
MagnificationNumerical
Aperture
ImmersionType
5x0.15Water, BABBHC PL FLUORTAR
10x0.30WaterHC APO L
20x0.50WaterHC APO L
DLS mirror (TwinFlect)DLS illumination
objective
DLS detection
objective
Immersion
2.5mm4x20xWater
5mm2.5x10x (or 20x)Water
7.8mm1.6x5x ( or 10x)Water
7.8mm1.6x5xBABB

Detectors

2x spectral PMT (Photo Multiplier Tube)
2x spectral HyD (Hybrid detector)
1x transmission PMT
1x scCMOS camera (Leica DFC9000 GTC DLS, 2048 x 2048 pixels)

Extra info

DLS emission band pass filter suitable for fluorophores excited with: 405nm, 488nm, 561nm and 633nm

Leica LAS-X software with:
Navigator package
LAS-X 3D viewer
LAS-X Live data mode

DMi8 inverted microscope stand with:
Automated motorized XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence.

Okolab stage top incubator for Z-Galvo stage with feedback sensor,
humidified 5% CO2 and objective heating.
Temperature data logging possible via LAS-X.
Okolab incubator inserts for 35mm & slide sized containers.

Applications

The DLS light sheet module from Leica (Digital Light Sheet), can be used to image specimen in the order from organoids to zebrafish embryo’s.
Live cell confocal imaging under CO2 and 37oC conditions
Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews
3D module for 3D rendering