Rescan confocal unit by confocal.nl with Nikon Ti inverted stand
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.45 | 4.00 | dry | Plan APO DIC |
| 60x | 1.45 | 0.13 | oil | Plan APO TIRF |
| 100x | 1.49 | 0.12 | oil | Plan APO TIRF |
| 100x | 1.40 | 0.13 | oil | Plan APO VC |
Pixel size (XY)
| Objective | Sweep factor 1 | Sweep factor 2 |
| 60x NA 1.45 | 144 nm | 72 nm |
| 100x NA1.49 | 84 nm | 42 nm |
Camera
Hamamatsu Orca Flash 4 scCMOS camera 2048×2048 pixels
Field of view per channel is 768 x 768 pixels
Extra info
Imaging under 5% CO2 and 30/37°C conditions, with stage top incubator, CO2 controller and objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
Hardware autofocus: Perfect Focus System (Nikon)
RCM unit (Confocal.nl) with “Churchwindow” emmision filter Blue/Green/Red/FarRed
Applications
ReScanConfocal Microscopy: for a higher resolution image with the optical sectioning of a confocal
Live cell confocal imaging under CO2 and 37°C conditions
Fixed cell imaging