Zeiss AxioImager II upright microscope
Spectrum
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 2.5x | 0.06 | 9.4 | Air | A-Plan, Pol |
| 5x | 0.12 | 9.9 | Air | A Plan, Pol |
| 10x | 0.25 | 6 | Air | N Achroplan |
| 20x | 0.5 | 2.0 | Air | EC Plan-NeoFluar, Pol |
| 40x | 0.47 | 0.47 | Air | Plan-NeoFluar, Pol |
| 63x | 0.95 | 0.12 | Air | EC Plan-Neofluar Korr., Pol |
Detectors
Axiocam MRm rev. 3 monochrome fluorescence camera
Axiocam 208 color camera for brightfield & polarisation acquisition
Filters
| Colour | Exitation | Emission | Type |
| Blue | BP 320-380 | BP 420-470 | DAPI, Hoechst |
| Cyan | BP 426-447 | BP 468-492 | Alexa Fluor 430, Aqua, DCC |
| Green | BP 477-500 | BP 514-540 | Alexa Fluor 488, FITC |
| Yellow | BP 523-548 | BP 564-595 | Alexa 532, Gold |
| Orange | BP 540-580 | BP 575-640 | Alexa Fluor 555, Cy3 |
| Red | BP 573-596 | BP 610-645 | Alexa Fluor 594, TexasRed |
| Far Red | BP 625-655 | BP655-715 | Alexa Fluor 647, Cy5 |
| Near-IR | BP 715-755 | BP 765-855 | Cy7 |
Extra info
XY stage with capability of loading up to 8 slides and tile-scan capabilities
Z-drive for z-stacks
Colibri 7 LED lightsource
Zen Blue imaging software
Applications
Widefield microscope with LED illumination system for automated and faster overview scans. Can be used before imaging samples on a confocal. The obtained overview can be used to locate area’s of interest to image with higher resolution and optical sectioning on a confocal microscope.