Axio Observer 7 microscope with SMART live cell imaging options
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance | Immersion | Type |
| 5x | 0.16 | 18.5mm | Dry | EC Plan-Neofluar |
| 10x | 0.3 | 5.2 | Dry | EC Plan-Neofluar |
| 20x | 0.8 | 0.55 | Dry | Plan-Apochromat |
| 25x | 0.8 | 0.57 | multi-Immersion | LD LCI Plan-Apochromat Corr DIC |
| 40x | 1.3 | 0.2 | Oil | Plan-Apochromat UV-IR |
| 40x | 1.1 | 0.62 | water | LD C-Apochromat |
| 63x | 1.4 | 0.2 | Oil | Plan-Apochromat |
Detectors
Airyscan 2; Patented superresolution imaging device (spectral range: 420-660)
2x PMT Spectral detection (spectral range: 405-700)
1x CH-GaAsP (spectral range: 405-700)
Transmitted light detector T-PMT 2
Filter
Filter set 90 HE LED
Excitation wavelengths: 385, 475, 555 and 630 nm.
Beam splitter QBS 405 + 493 + 575 + 653
Emission filter QBP 425/30 + 514/30 + 592/25 + 709/100
Extra information
Light Source Viluma 5 RGB-UV for EPI Fluorescence
HAL 100/LED for brightfield imaging
Axiocam 305 mono Camera
AI Sample Finder for quick tissue overviews
ZEN Blue software with Toolkits for:
Advanced SMART Live cell 2D/3D Acquisition
ZEN Developer to automate image acquisition, image processing and analysis steps.
ZEN Dynamics Profiler Advanced; allows to gather details of molecular characteristics of living sampes, providing concentration and dynamics information of molecules (e.g FRAP)
Ibidi™ Temperature and humidified CO2/O2 Controler with heated plate, glass lid and objective heater and Ibidi™ IncuControl software.
Inserts for: 35mm, 60mm dishes, (chamber) slides and multiwell plates.
Applications
This microscope is especially equipped for SMART live cell confocal imaging under CO2 and 37°C conditions.
SMART set-up for well plate imaging with long distance objectives and auto immersion of water to provide stable imaging.
FRAP, FRET and advanced multi location time lapse imaging
Fixed cell imaging
Tiling module for fast overviews