Leica SP5 laser scanning confocal with AOBS
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.40 | 2.20 | dry | HCX PL APO CS |
| 20x | 0.70 | 0.59 | dry | HCX PL APO CS |
| 20x | 0.70 | 0.25 | water/glyc./oil | HCX PL Fluotar L |
| 40x | 1.25 | 0.10 | oil | HCX PL APO CS |
| 63x | 1.40 | 0.14 | oil | HCX PL APO CS |
Detectors
3x spectral PMT detector (Photo Multiplier Tube)
2x spectral APD detector (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization
Extra info
Leica LAS-AF software with:
Matrix screening software for high content screening
FRAP & FRET modules
Motorized stage with inserts for slides, 35mm dishes, and multiwell plates
Metal-Halide lamp for EPI fluorescence
Avalance photo diodes for very high sensitivity
Peecon small chamber incubator with heating, CO2 and O2 regulation
Applications
Live cell confocal imaging under CO2 and 37oC conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging