Leica SP5 AOBS Multi-photon lasers
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.40 | 2.2 | dry | HCX PL APO CS |
| 20x | 0.70 | 0.59 | dry | HCX PL APO CS |
| 40x | 1.25 | 0.10 | oil | HCX PL APO CS |
| 63x | 1.40 | 0.14 | oil | HCX PL APO CS |
| 100x | 1.35 | – | glycerol | – |
Detectors
3x spectral PMT detector (Photo Multiplier Tube)
2x spectral APD detector (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization
Extra info
Multiphoton laser Coherent Chameleon Ultra (710 – 1040nm)
266nm UV laser
Avalanche photodiodes for extremely low signals
Leica LAS-AF software with:
FRAP & FRET modules
Live data mode
Matrix screening software for high content screening
Leica DMi6000 inverted microscope stand with:
Motorized stage with inserts for slides, 35mm dishes, and multiwell plates
Peecon small chamber incubator with heating, CO2 and O2 regulation
Metal-Halide lamp for EPI fluorescence
Applications
This microscope is especially equipped for making localized DNA damage and/or multiphoton imaging.
Live cell confocal imaging under CO2 and 37oC conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging