Nikon Ti-Eclipse inverted microscope with Spinning disc unit and QuantEM EMCCD camera
Lasers
Objectives
| Magnification | Pixel size (nm) | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 20x | 330 | 0.75 | 1.00 | dry | Plan APO DIC |
| 40x | 164 | 1.30 | 0.20 | oil | Plan APO DIC |
| 63x | 112 | 1.40 | 0.13 | oil | Pan APO VC |
| 100x | 67 | 1.49 | 0.12 | oil | Plan APO TIRF |
Camera
EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)
Filters
| Colour | Exitation | Emission | Type |
| Blue | 350/50x | 460/40m | Chroma 49000 ET-DAPI |
| Green | 470/40x | 525/50m | Chroma 49002 ET-GFP |
| Red | 560/40x | 630/75m | Chroma 49008 ET-mCh/tR |
| Green + Red | 450-490 + 550-590 | 525/25m + 630/30m | Chroma 59022 ET-GFP/mCherry |
Extra info
Imaging under CO2 and 30/37oC conditions, with stage top incubator, CO2 controller, objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
Spinning Disc unit, CSU-X1 (Yokogawa)
FRAP3D unit: ultrafast by Galvo mirror positioned laserbleaching (ROPER)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green/Red or Cyan/Green
Piëzo stage for fast Z-stacks
Hardware autofocus: Perfect Focus System (Nikon)
Metamorph Imaging Software
Applications
Fast Live cell confocal like imaging, with less monitor bleaching (in vitro or in vivo)
Live Cell imaging imaging conditions.
Multichannel 4D imaging (3D timelapse imaging)
Imaging activatable or switchable fluorophores
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Fixed cell imaging