Zeiss Elyra PS1 SIM, PALM modules and LSM 780 confocal
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.3 | 5.2 | dry | Plan NeoFluar |
| 63x | 1.4 | 0.4 | oil | Plan Apochromat DIC |
| 100x | 1.46 | 0.11 | oil | α Plan Apochromat DIC |
| 100x | 1.57 | 0.11 | oil | Korr α Plan Apochromat |
Detectors
Confocal
32 channel GaAsP (spectral range 412nm – 691nm) (Gallium Arsenide Phosphide detector)
Blue PMT (spectral range 371nm – 740nm) (Photo Multiplier Tube)
Red PMT (spectral range 379nm – 758nm) (Photo Multiplier Tube)
SIM
EMCCD camera Andor iXon DU 885, 1002×1004 pixels
Single Molecule imaging
EMCCD camera Andor iXon DU 897, 512x512pixels
Filters SIM/PALM
Fset 77 HE (3 colour triple filter green, red, far red) only filter for visual inspection
BP 420-480 + LP 750, blue dyes
BP 495-575 + LP 750, green dyes
BP 570-650 + LP 750, red dyes
LP 655 , far red dyes
Lasers
Confocal
405 nm – 30mW
458 nm, 488 nm, 514 nm – Argon
561 nm – 10mW
633 nm – HeNe
SIM/PALM
405 nm – 50mW
488 nm – 100mW
561 nm – 100mW
642 nm – 100mW
Extra info
Mercury lamp for EPI fluorescence: Excelitas X-CITE® 120
Piezo stage
Tokai Hit lightweight stage top incubator for CO2 and 37oC conditions with inserts for slides and 35mm dishes with feedback sensor, humidified CO2 controller and objective heating.
Zeiss Zen blue SIM reconstruction module
Zeiss Zen blue Single molecule analysis module
Applications
Structured Illumination Microscopy (SIM)
Stochastic Optical Reconstruction Microscopy (STORM)
Photo Activation Localization Microscopy (PALM)
Single molecule localization microscopy (SMLM)
Live cell confocal imaging under CO2 and 37oC conditions FRAP, FRET, Time lapse imaging
Fixed cell imaging