Leica SP5 confocal with AOBS, GalvoZ stage and Adaptive Focus Control (AFC)
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 5x | 0.15 | 13.7 | dry | HCX PL Fluotar |
| 10x | 0.40 | 2.2 | dry | HCX PL APO CS |
| 20x | 0.70 | 0.59 | dry | HCX PL APO CS |
| 20x | 0.70 | 0.25 | water/glyc./oil | HCX PL APO CS |
| 40x | 1.25 | 0.10 | oil | HCX PL APO CS |
| 63x | 1.40 | 0.14 | oil | HCX PL APO CS |
Detectors
2x spectral PMT (Photo Multiplier Tube)
1x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization
Extra info
Metal-Halide lamp for EPI fluorescence
GalvoZ stage with inserts for slides, 35mm dishes, 10cm dishes, and multi-well plates Adaptive Focus Control
Large incubator with heating and CO2
Matrix screening software for high content screening
Applications
Live cell confocal imaging under CO2 and 37oC conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging