Leica SP5 confocal with AOBS, GalvoZ stage and Adaptive Focus Control (AFC)
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 5x | 0.15 | 13.7 | dry | HCX PL Fluotar |
| 10x | 0.40 | 2.2 | dry | HCX PL APO CS |
| 20x | 0.70 | 0.59 | dry | HCX PL APO CS |
| 20x | 0.70 | 0.25 | water/glyc./oil | HCX PL APO CS |
| 40x | 0.60 | 1.9-3.3 | dry | HCX PL Fluotar L |
| 40x | 1.25 | 0.10 | oil | HCX PL APO CS |
| 63x | 1.40 | 0.14 | oil | HCX PL APO CS |
Detectors
2x spectral PMT (Photo Multiplier Tube)
1x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization
Extra info
Leica LAS-AF software with:
Matrix screening software for high content screening
FRAP & FRET modules
Live data mode
Leica DMi6000 inverted microscope stand with:
Adaptive Focus Control
GalvoZ stage with inserts for slides, 35mm dishes, 10cm dishes, and multi-well plates
Large incubator with heating and CO2
Metal-Halide lamp for EPI fluorescence
Applications
Live cell confocal imaging under 5% CO2 and 37oC conditions, also for multi-well plates.
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging