Leica SP5 604 / Ee-604

Leica SP5 confocal with AOBS, GalvoZ stage and Adaptive Focus Control (AFC)

Lasers

Objectives

MagnificationNumerical
Aperture
Working
Distance (mm)
ImmersionType
5x0.1513.7dryHCX PL Fluotar
10x0.402.2dryHCX PL APO CS
20x0.700.59dryHCX PL APO CS
20x0.700.25water/glyc./oilHCX PL APO CS
40x0.601.9-3.3dryHCX PL Fluotar L
40x1.250.10oilHCX PL APO CS
63x1.400.14oilHCX PL APO CS

Detectors

2x spectral PMT (Photo Multiplier Tube)
1x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization

Extra info

Leica LAS-AF software with:
Matrix screening software for high content screening
FRAP & FRET modules
Live data mode

Leica DMi6000 inverted microscope stand with:
Adaptive Focus Control
GalvoZ stage with inserts for slides, 35mm dishes, 10cm dishes, and multi-well plates
Large incubator with heating and CO2
Metal-Halide lamp for EPI fluorescence

Applications

Live cell confocal imaging under 5% CO2 and 37oC conditions, also for multi-well plates.
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging