Leica SP5 604 / Ee-604

Leica SP5 confocal with AOBS, GalvoZ stage and Adaptive Focus Control (AFC)

Lasers

Objectives

MagnificationNumerical
Aperture
Working
Distance (mm)
ImmersionType
5x0.1513.7dryHCX PL Fluotar
10x0.402.2dryHCX PL APO CS
20x0.700.59dryHCX PL APO CS
20x0.700.25water/glyc./oilHCX PL APO CS
40x1.250.10oilHCX PL APO CS
63x1.400.14oilHCX PL APO CS

Detectors

2x spectral PMT (Photo Multiplier Tube)
1x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization

Extra info

Metal-Halide lamp for EPI fluorescence
GalvoZ stage with inserts for slides, 35mm dishes, 10cm dishes, and multi-well plates Adaptive Focus Control
Large incubator with heating and CO2
Matrix screening software for high content screening

Applications

Live cell confocal imaging under 5% CO2 and 37oC conditions, also for multi-well plates.
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging