Leica SP5 AOBS Multi-photon lasers
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.40 | 2.2 | dry | HCX PL APO CS |
| 20x | 0.70 | 0.59 | dry | HCX PL APO CS |
| 40x | 0.60 | 1.9-3.3 | dry | HCX PL Fluotar L |
| 40x | 1.25 | 0.10 | oil | HCX PL APO CS |
| 63x | 1.40 | 0.14 | oil | HCX PL APO CS |
| 100x | 1.35 | – | glycerol | – |
Detectors
3x spectral PMT detector (Photo Multiplier Tube)
2x spectral APD detector (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization
Extra info
Coherent Chameleon Ultra (710 – 1040nm)
266nm UV laser
Metal-Halide lamp for EPI fluorescence
Motorized stage with inserts for slides, 35mm dishes, and multiwell plates
Peecon small chamber incubator with heating, CO2 and O2 regulation
Matrix screening software for high content screening
Avalanche photodiodes for extremely low signals
Applications
This microscope is especially equipped for making localized DNA damage and/or multiphoton imaging.
Live cell confocal imaging under CO2 and 37oC conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging