Leica SP5 778 / Ee-778

Leica SP5 AOBS Multi-photon lasers

Lasers

Objectives

MagnificationNumerical
Aperture
Working
Distance (mm)
ImmersionType
10x0.402.2dryHCX PL APO CS
20x0.700.59dryHCX PL APO CS
40x0.601.9-3.3dryHCX PL Fluotar L
40x1.250.10oilHCX PL APO CS
63x1.400.14oilHCX PL APO CS
100x1.35glycerol

Detectors

3x spectral PMT detector (Photo Multiplier Tube)
2x spectral APD detector (Avalanche Photo Diode)
1x transmission PMT for bright field, DIC and polarization

Extra info

Coherent Chameleon Ultra (710 – 1040nm)
266nm UV laser
Metal-Halide lamp for EPI fluorescence
Motorized stage with inserts for slides, 35mm dishes, and multiwell plates
Peecon small chamber incubator with heating, CO2 and O2 regulation
Matrix screening software for high content screening
Avalanche photodiodes for extremely low signals

Applications

This microscope is especially equipped for making localized DNA damage and/or multiphoton imaging.
Live cell confocal imaging under CO2 and 37oC conditions
FRAP, FRET, (advanced multi well)
Time lapse imaging
Fixed cell imaging