Leica SP8 CIL / Ee-164

Leica SP8 Low Incidence Angle optics, C1 lab, Galvo Z stage & Adaptive Focus Control

Lasers

Objectives

MagnificationNumerical
Aperture
Working
Distance (mm)
ImmersionType
10x0.402.20AirHC PL APO CS2
20x0.750.62AirHC PL APO CS2
40x1.300.24OilHC PL APO CS2
63x1.400.14OilHC PL APO CS2

Detectors

2x spectral PMT (Photo Multiplier Tube)
1x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization

Extra info

Leica LAS-X software with navigator package
Metal-Halide lamp for EPI fluorescence
Galvo Z stage with inserts for slides & 35mm containers
Okolab cage incubator with feedback sensor, humidified 5% CO2.
Temperature data logging possible via LAS-X.
Okolab incubator inserts for 35mm, slide sized containers & multi-well plates.
Adaptive Focus Control.
DMi8 inverted microscope stand with automated XY stage.

Applications

This microscope is situated in an C1 lab for confocal imaging experiments combined with radioactivity

Live cell confocal imaging under CO2 and 37oC conditions Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews