Leica SP8 Low Incidence Angle optics, C1 lab, Galvo Z stage & Adaptive Focus Control
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.40 | 2.20 | Air | HC PL APO CS2 |
| 20x | 0.75 | 0.62 | Air | HC PL APO CS2 |
| 40x | 1.30 | 0.24 | Oil | HC PL APO CS2 |
| 63x | 1.40 | 0.14 | Oil | HC PL APO CS2 |
Detectors
2x spectral PMT (Photo Multiplier Tube)
1x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization
Extra info
Leica LAS-X software with navigator package
DMi8 inverted microscope stand with:
Automated XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence
Okolab cage incubator around microscope stand with:
Okolab incubator inserts for 35mm, slide sized containers & multi-well plates.
For 37°C conditions with humidified 5% CO2.
Temperature data logging possible via LAS-X.
Applications
This microscope is situated in an C1 lab for confocal imaging experiments combined with radioactivity
Live cell confocal imaging under CO2 and 37oC conditions Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews