Leica SP8 / Ee-775a

Leica SP8 AOBS, UV laser by Rapp OptoElectronics, Galvo Z stage & Adaptive Focus Control (AFC)

Lasers

Objectives

MagnificationNumerical
Aperture
Working
Distance (mm)
ImmersionType
10x0.402.20AirHC PL APO CS2
20x0.750.62AirHC PL APO CS2
40x1.300.24OilHC PL APO CS2
63x1.400.14OilHC PL APO CS2
40x0.600.36GlycerolZeiss Ultrafluar

Detectors

2x spectral PMT (Photo Multiplier Tube)
2x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization

Extra info

Leica LAS-X software with navigator package
Leica Lightning deconvolution
Metal-Halide lamp for EPI fluorescence
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Okolab stage top incubator for Z-Galvo stage with feedback sensor, humidified 5% CO2 and objective heating.
Temperature data logging possible via LAS-X.
Okolab incubator inserts for 35mm & slide sized containers
Deep UV (266nm & 355nm) system by Rapp OptoElectronics for DNA damage optogenetics by laser scanning unit
DMi8 inverted microscope stand with automated XY stage

Applications

This microscope is especially equipped for making localized DNA damage
Live cell confocal imaging under CO2 and 37°C conditions
FRAP, FRET, (advanced multi location) time lapse imaging
Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews