Leica SP8 AOBS, UV laser by Rapp OptoElectronics, Galvo Z stage & Adaptive Focus Control (AFC)
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.40 | 2.20 | Air | HC PL APO CS2 |
| 20x | 0.75 | 0.62 | Air | HC PL APO CS2 |
| 40x | 1.30 | 0.24 | Oil | HC PL APO CS2 |
| 63x | 1.40 | 0.14 | Oil | HC PL APO CS2 |
| 40x | 0.60 | 0.36 | Glycerol | Zeiss Ultrafluar |
Detectors
2x spectral PMT (Photo Multiplier Tube)
2x spectral HyD (Hybrid detector)
1x transmission PMT for brightfield, DIC and polarization
Extra info
Deep UV (266nm & 355nm) system by Rapp OptoElectronics for DNA damage optogenetics by laser scanning unit
Leica LAS-X software with:
Navigator package
LAS-X Live data mode
FRAP module
Leica Lightning deconvolution
DMi8 inverted microscope stand with:
Automated XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence
Okolab stage top incubator for Z-Galvo stage with feedback sensor,
humidified 5% CO2 and objective heating.
Temperature data logging possible via LAS-X.
Okolab incubator inserts for 35mm & slide sized containers.
Applications
This microscope is especially equipped for making localized DNA damage
Live cell confocal imaging under CO2 and 37°C conditions
FRAP, FRET, (advanced multi location) time lapse imaging
Fixed cell imaging
Lightning module for real-time deconvolution
Navigator module for fast overviews