Leica SP8 AOBS with gated 3D tauSTED, white light laser, lightning and FALCON
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.40 | 2.20 | Air | HC PL APO CS2 |
| 20x | 0.75 | 0.62 | Air | HC PL APO CS2 |
| 40x | 1.30 | 0.24 | Oil | HC PL APO CS2 |
| 63x | 1.40 | 0.14 | Oil | HC PL APO CS2 |
| 100x | 1.40 | Oil | HC PL APO STED | |
| 86x | 1.20 | Water | HC PL APO STED |
Detectors
2x spectral PMT (Photo Multiplier Tube)
2x gated spectral GaAsP HyD (Hybrid detector for gated imaging)
1x transmission PMT for brightfield, DIC and polarization
Extra info
3x STED depletion lasers: 592, 660 & 775nm
Leica LAS-X software with:
Navigator package
LAX-X 3D viewer
Leica Lightning deconvolution
DMi8 inverted microscope stand with:
Automated motorized XY stage
Galvo Z stage with inserts for slides & 35mm containers
Adaptive Focus Control
Metal-Halide lamp for EPI fluorescence.
Tokai Hit stage top incubator for Z-Galvo stage with
humidified 5% CO2 and objective heating.
Stage top incubator inserts for 35mm & slide sized containers.
Applications
Super resolution imaging with (3D) STED, combined with lifetime; Tau-STED
Confocal imaging combined with lifetime imaging; FALCON
Confocal imaging combined with deconvolution; Lightning
Spectral freedom with White Light Laser 470-670nm range
Navigator module for fast overviews