Nikon Spinning Disc 1454 / Ee-1454

Nikon Ti-Eclipse inverted microscope with Spinning disc unit and QuantEM EMCCD camera

Lasers

Objectives

MagnificationPixel size (nm)Numerical
Aperture
Working
Distance (mm)
ImmersionType
20x3300.751.00dryPlan APO DIC
40x1641.300.20oilPlan APO DIC
63x1121.400.13oilPan APO VC
100x671.490.12oilPlan APO TIRF

Camera

EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)

Extra info

Cell culturing equipment: Small chamber water bath incubator with CO2 regulation (Tokai Hit)
Spinning Disc unit, CSU-X1 (Yokogawa)
FRAP3D unit: ultrafast by Galvo mirror positioned laserbleaching (ROPER)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green/Red or Cyan/Green
Piƫzo stage for fast Z-stacks
Hardware autofocus: Perfect Focus System (Nikon)
Metamorph Imaging Software

Applications

Fast Live cell confocal like imaging, with less monitor bleaching (in vitro or in vivo)
Imaging under CO2 and 37oC conditions
Multichannel 4D imaging (3D timelapse imaging)
Imaging activatable or switchable fluorophores
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Fixed cell imaging