Nikon Spinning Disc 1454 / Ee-1454

Nikon Ti-Eclipse inverted microscope with Spinning disc unit and QuantEM EMCCD camera



MagnificationPixel size (nm)Numerical
Distance (mm)
20x3300.751.00dryPlan APO DIC
40x1641.300.20oilPlan APO DIC
63x1121.400.13oilPan APO VC
100x671.490.12oilPlan APO TIRF


EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)

Extra info

Imaging under CO2 and 30/37oC conditions, with stage top incubator, CO2 controller, objective heating (Tokai Hit).
Tokai Hit inserts for 35mm and objective slide container available.
Spinning Disc unit, CSU-X1 (Yokogawa)
FRAP3D unit: ultrafast by Galvo mirror positioned laserbleaching (ROPER)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green/Red or Cyan/Green
Piƫzo stage for fast Z-stacks
Hardware autofocus: Perfect Focus System (Nikon)
Metamorph Imaging Software


Fast Live cell confocal like imaging, with less monitor bleaching (in vitro or in vivo)
Live Cell imaging imaging conditions.
Multichannel 4D imaging (3D timelapse imaging)
Imaging activatable or switchable fluorophores
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Fixed cell imaging