Nikon TIRF 1454 / Ee-1454

Nikon Ti-Eclipse inverted microscope with TIRF unit and QuantEM EMCCD camera

Lasers

Objectives

MagnificationPixel size (nm)Numerical
Aperture
Working
Distance (mm)
ImmersionType
10x4300.454.00dryPlan APO DIC
60x1111.450.13oilPan APO TIRF
100x671.490.12oilPlan APO TIRF
100x671.400.13oilPlan APO VC

Camera

EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)

Extra info

Cell culturing equipment: Small chamber water bath incubator with CO2 regulation (Tokai Hit)
TIRF mount for manual or mechanic TIRF angle control
FRAP3D unit: ultrafast by galvo mirror positioned laserbleaching (ROPER)
Hardware autofocus: Perfect Focus System (Nikon)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green/Red or Cyan/Green
Metamorph Imaging Software

Applications

Live cell TIRF/WideField imaging under CO2 and 37oC conditions
Membrane studies (in vitro & in vivo)
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Single Molecule Imaging, PALM and STORM
Imaging activatable or switchable fluorophores
Fixed cell imaging