Nikon Ti-Eclipse inverted microscope with TIRF unit and QuantEM EMCCD camera
Lasers
Objectives
| Magnification | Pixel size (nm) | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 430 | 0.45 | 4.00 | dry | Plan APO DIC |
| 60x | 111 | 1.45 | 0.13 | oil | Pan APO TIRF |
| 100x | 67 | 1.49 | 0.12 | oil | Plan APO TIRF |
| 100x | 67 | 1.40 | 0.13 | oil | Plan APO VC |
Camera
EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)
Extra info
Cell culturing equipment: Small chamber water bath incubator with CO2 regulation (Tokai Hit)
TIRF mount for manual or mechanic TIRF angle control
FRAP3D unit: ultrafast by galvo mirror positioned laserbleaching (ROPER)
Hardware autofocus: Perfect Focus System (Nikon)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green/Red or Cyan/Green
Metamorph Imaging Software
Applications
Live cell TIRF/WideField imaging under CO2 and 37oC conditions
Membrane studies (in vitro & in vivo)
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Single Molecule Imaging, PALM and STORM
Imaging activatable or switchable fluorophores
Fixed cell imaging