Zeiss Elyra PS1 / Be-311

Zeiss Elyra PS1 SIM, PALM modules and LSM 780 confocal

Lasers

Objectives

MagnificationNumerical
Aperture
Working
Distance (mm)
ImmersionType
10x0.35.2dryPlan NeoFluar
63x1.40.4oilPlan Apochromat DIC
100x1.460.11oilα Plan Apochromat DIC
100x1.570.11oilKorr α Plan Apochromat

Detectors

Confocal
32 channel GaAsP (spectral range 412nm – 691nm) (Gallium Arsenide Phosphide detector)
Blue PMT (spectral range 371nm – 740nm) (Photo Multiplier Tube)
Red PMT (spectral range 379nm – 758nm) (Photo Multiplier Tube)

SIM
EMCCD camera Andor iXon DU 885, 1002×1004 pixels

Single Molecule imaging
EMCCD camera Andor iXon DU 897, 512x512pixels

Filters SIM/PALM

Fset 77 HE (3 colour triple filter green, red, far red) only filter for visual inspection
BP 420-480 + LP 750, blue dyes
BP 495-575 + LP 750, green dyes
BP 570-650 + LP 750, red dyes
LP 655 , far red dyes

Lasers

Confocal
405 nm – 30mW
458 nm, 488 nm, 514 nm – Argon
561 nm – 10mW
633 nm – HeNe

SIM/PALM
405 nm – 50mW
488 nm – 100mW
561 nm – 100mW
642 nm – 100mW

Extra info

Mercury lamp for EPI fluorescence 
Piezo stage
Tokai Hit lightweight stage top incubator for CO2 and 37oC conditions with inserts for slides and 35mm dishes with feedback sensor, humidified CO2 controller and objective heating.
Zeiss Zen blue SIM reconstruction module
Zeiss Zen blue Single molecule analysis module 

Applications

Structured Illumination Microscopy (SIM)
Stochastic Optical Reconstruction Microscopy (STORM)
Photo Activation Localization Microscopy (PALM)
Single molecule localization microscopy (SMLM)

Live cell confocal imaging under CO2 and 37oC conditions FRAP, FRET, Time lapse imaging
Fixed cell imaging