Zeiss LSM 510 confocal with Meta spectral detector, Axiovert 200M inverted microscope
Lasers
Objectives
Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
10x | 0.3 | 5.5 | dry | Plan-NeoFluar |
20x | 0.5 | 2.0 | dry | Plan-NeoFluar |
40x | 1.3 | 0.21 | oil | Plan-NeoFluar |
63x | 1.4 | 0.09 | oil | Plan-Apochromat |
100x | 1.2 | – | glycerol | Ultrafluar (266nm) |
Detectors
1x spectral Meta detector
2x spectral PMT detector (Photo Multiplier Tube)
1x transmission light detector
Extra info
Mercury lamp for EPI fluorescence
Automatic scanningstage
Large incubator with humidified
CO2 266nm UV laser for DNA damage
LSM510 version 3.2 AIM software
Applications
Live cell confocal imaging with large incubator under CO2 and 37oC conditions
FRAP, FRET, Time lapse imaging
DNA damage induction
Fixed cell imaging