Zeiss LSM 510 confocal with Meta spectral detector, Axiovert 200M inverted microscope
Lasers
Objectives
| Magnification | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 0.3 | 5.5 | dry | Plan-NeoFluar |
| 20x | 0.5 | 2.0 | dry | Plan-NeoFluar |
| 40x | 1.3 | 0.21 | oil | Plan-NeoFluar |
| 63x | 1.4 | 0.09 | oil | Plan-Apochromat |
| 40x | 0.8 | 2 | water | Achroplan (dipping) |
Detectors
1x spectral Meta detector
2x spectral PMT detector (Photo Multiplier Tube)
1x transmission light detector
Extra info
Mercury lamp for EPI fluorescence
System is suited for long term timelapse imaging
Applications
Long term live cell confocal imaging under CO2 and 37oC conditions
FRAP, GFP-mRFP acceptor photo bleaching FRET
Imaging of UV dyes
Fixed cell imaging