Zeiss Near-infrared Wide Field / Ee-775a

Axio Imager D2 upright microscope

Spectrum

Objectives

MagnificationNumerical
Aperture
Working
Distance (mM)
ImmersionType
5x0.1618.5dryEC Plan-NeoFluar
10x0.35.2dryEC Plan-NeoFluar
20x0.52.0dryEC Plan-NeoFluar
40x0.750.71dryEC Plan-NeoFluar
63x1.400.19oilPlan-Apochromat DIC

Detectors

Coolsnap MYO, CCD camera with 1940 x 1460 pixels, 14bit

Filters

ColourExitationEmissionType
BlueBP 335-383BP 420-470Dapi
GreenBP 450-490LP 51509 Alexa Fluor 488
RedBP 538-562BP 570-64043 HE DsRed (Cy3)
Far Red (Cy5)BP 625-655BP 665-71550 Cy5
Far Red (Cy5.5)BP 643-688BP700-75032 Alexa Fluor 680 (Cy5.5)
Near Infra RedBP 665-745BP 755-855Cy7

Extra info

Metal Halide PhotoFluor LM-75 LED (340 nm – 800 nm)
Collimated 730nm LED used for NIR range in combination with Cy7 filter (730nm, 195mW)
Zen Blue imaging software

Applications

Near-infrared imaging