Optical Imaging and live cell microscopy

MSc Course (October 7-11, 2024)

This course is organised in collaboration with the Infection & Immunity, Neuroscience and Molecular Medicine research master schools. The course is open for master students of Infection & Immunity, Neuroscience and Molecular Medicine research master schools (ECTS 1.8).

The one week course consists of lectures in the morning and hands-on practical sessions in the afternoon. The practical exercises at the confocal microscopes are performed in groups of max. 5 participants.

Topics

  • Basal microscopy, Confocal microscopy, GFP and live cell imaging, Fluorescent recovery after photobleaching (FRAP)
  • Fluorescent resonance energy transfer (FRET)
  • Super resolution (SIM/STED, single molecule localization (PALM/STORM))
  • Image analysis

The course will be concluded with groups of students giving a presentation of the results from one of the course topics.

Registration

Registration through your own research school (preferred) or by sending an email to Martijn de Gruiter with your name and masterschool. The maximum number of participants is 25

Organisers: Laurens Bosman, Gerard Borst, Gert-Jan Kremers, Adriaan Houtsmuller Martijn de Gruiter and Gert van Cappellen

Course material

Participants of the course can download the files needed for the course via the link below. This includes the program of the lectures and practical sessions, manuals for the different practicals and images needed for the Fiji workshop. At the end of the course this link will be updated with presentations of the lectures given during this course.