Functional Imaging and Super Resolution

PhD Course November 8-12, 2021

The course is cancelled

This intensive course of one week gives an overview of the rapidly developing advanced fluorescence imaging methods and how they are used in biomedical research. An important part is the practical work each afternoon in which you get the opportunity for real hands-on experience. The focus of the practicals is on functional imaging (time lapse, FRAP, FRET) and super resolution (SIM, STED and single molecule tracking).

This five day course consists of lecture sessions in the morning and practical sessions in the afternoon. On monday and thursday there are also evening sessions with dinner. In the final session on friday afternoon participants present and discuss the results of one of the topics during the practical work. The practical sessions can be attended by a maximum of 25 participants (5 groups of 5 participants).

Lecture Topics

Confocal Microscopy & Live Cell Imaging, FRET, FRAP, TIRF, FLIM, Super resolution (SIM, STED, PALM/STORM, Rescan), Deconvolution,  Fluorescent Proteins, Biological Applications and Image Analysis 

Hands-on Practical Work

Introduction to Confocal Microscopy, Time Lapse Imaging, FRAP,  Super resolution Imaging (SIM, STED, PALM/STORM/Localisation Mic., Rescan, lightsheet), data analysis, data presentation.

The course is intended for PhD students and post-docs and others who need more background in advanced fluorescent imaging and data analysis.

No prior knowledge is required.