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Demo Imaging Facility Microscope

Abbelight TIRF MN360 Demo

Multicolor, Single molecule localization (dSTORM, PALM, and PAINT) and TIRF microscopy

We are excited to announce that our imaging facility has a state-of-the-art Abbelight MN360 system available for demonstation from July 7 till 11.

The Erasmus Optical Imaging Centre together with Abbelight will host a SAFe MN630 demonstration. This add on module is used for multicolor Total Internal Reflection Fluorescence (TIRF) that exploits the unique properties of an induced evanescent wave in a limited specimen region immediately adjacent to the interface between two media having different refractive indices (e.g.: the contact area between a specimen and a glass coverslip).

The MN360 offers an optimal and simultaneous multicolor imaging with the benefits of ASTER technology. This technology provides an ultra-widefield and speckle-free illumination of biological structures close to the coverslip such as membranes, focal adhesions, and much more.  

For more information on the system visite the Evident website: https://evidentscientific.com/en/products/super-resolution/abbelight

For more information on the demonstration please contact Gert-Jan Kremers

Categories
Microscope OIC News

Evident FV4000 confocal demonstration

From February 5th to 9th 2024 Evident (formerly Olympus), in association with the OIC, will organize a demonstration of the newly released Fluoview FV4000 point scanning confocal. 

Researchers who are interested to test the possibilities of this new generation laser scanning confocal are invited to bring their own samples for testing. 

The FV4000 can image most of the regular samples used on the current OIC confocals. In addition, the confocal setup should be especially interesting for researchers using near infrared (NIR) fluorescent probes, or more than 4 fluorescent stains. 

Special objectives will be available for imaging organoids and tissues. 
Specifications of the demonstration setup include: 

  • Inverted microscope 
  • 10 laser spanning the wavelength range from 405 nm – to 785 nm 
  • 6 detectors for simulaneous imaging and spectral unmixing 
  • Incubation system for live cell imaging (37°C/5% CO2) using 35mm dishes, multiwell slides and multiwell dishes 
  • Resonant scanner for fast (>20fps) imaging 

If you are interested to test the FV4000, please send us an email with a short description of the sample you would like to image. 

Categories
Microscope OIC News

New microscope installed: Zeiss LSM900

In december the new Zeiss LSM900 is in Be-311 will be available for use. After the installation this summer we have fully tested and optimized the system for our imaging facility.

About the microscope

This microscope is also for fixed specimens, has 3 detectors and has the Zen-blue 3.7 software with an extended tile-scan module.

LSM700 users who did the introduction course can have an extra short (1 hour) introduction on the new microscope, mostly to show the differences and extended possibilities compared to the lsm700. Extra manuals will be provided via our website. Both the LSM700 systems will remain in our imaging facility.