Tsion, Martijn, Johan,
Marla, Bart, Ilse, Gert-Jan, Alex,
Adriaan & Gert
Confocal image (circular crop) of a fertilized mouse oocyte, with male and female pronuclei (yellow) and four prominent nucleoli (red) in the female pronucleus. The oocyte was decorated with ‘candle lights’ which are copies from the rightmost bright tiny structure in the cytoplasm (original image recorded by Gert van Cappellen, OIC)
The Erasmus Optical Imaging Centre will host a MinFlux demonstration in the period of November 11-15 and a NVvM workshop focussed on MinFlux microscopy on Monday November 18.
MINFLUX, or minimal fluorescence photon fluxes microscopy, is a super-resolution light microscopy method that offers nanometer-scale 3D-imaging and microsecond-range single molecule tracking and can attain 1–3 nm resolution in three dimensions
We are excited to announce that our imaging facility has a state-of-the-art Abberior MinFlux system available for demonstation November 11-15. The system will also be part of the OIC functional imaging course in the same week.
The demonstration will be concluded with a whole day NVvM MinFlux workshop on Monday November 18, 2024.
More information about the workshop, demonstration period and the registration can be found here.
We would like to introduce our new colleague Ilse Bakker, who started Juny of this year.
Ilse will be working in the OIC in the context of a project financed by the Convergence Flagship Imaging Facility and Innovation Centre (CIFIC) lead by Adriaan Houtsmuller, Erasmus MC and Jacob Hoogeboom, TU Delft. She will develop novel methods and staining techniques for combining fluorescent confocal microscopy and electron microscopy.
Registration is not longer possible for our PhD functional imaging and super-resolution course on November 11-15 2024.
The course is full, your registration will be placed on a waiting list. The course will be organized again in the fall of 2025.
This intensive course of one week gives an overview of the rapidly developing advanced fluorescence imaging methods and how they are used in biomedical research in a combination of lectures and hands-on practicals at our microscopes.
More information about the course and the registration form can be found via this link.
On Monday and Tuesday Jan 29th-30th 2024 the OIC organizes in cooperation with the Erasmus MC graduate school the two-day Image analysis course “Microscopic Image Analysis: From Theory to Practice”. This course focuses on first time users and explains the basis of image analysis and how to use this in Fiji (ImageJ).
Microscopic images contain much more information than normally is retrieved. Extracting this information by visual inspection and manual measurement is not only cumbersome but also subjective. Automation of image analysis tasks by using a computer and the right software tools allows for higher efficiency, accuracy, objectivity, reproducibility, and completeness.
Participants should bring their own laptop to the course for the practical exercises. Software and sample image data will be provided during the course. No prior knowledge of image processing is required. Participants have the opportunity to bring their own image data and directly apply the newly acquired image analysis techniques.
From February 5th to 9th 2024 Evident (formerly Olympus), in association with the OIC, will organize a demonstration of the newly released Fluoview FV4000 point scanning confocal.
Researchers who are interested to test the possibilities of this new generation laser scanning confocal are invited to bring their own samples for testing.
The FV4000 can image most of the regular samples used on the current OIC confocals. In addition, the confocal setup should be especially interesting for researchers using near infrared (NIR) fluorescent probes, or more than 4 fluorescent stains.
Special objectives will be available for imaging organoids and tissues.
Specifications of the demonstration setup include:
If you are interested to test the FV4000, please send us an email with a short description of the sample you would like to image.
In december the new Zeiss LSM900 is in Be-311 will be available for use. After the installation this summer we have fully tested and optimized the system for our imaging facility.
This microscope is also for fixed specimens, has 3 detectors and has the Zen-blue 3.7 software with an extended tile-scan module.
LSM700 users who did the introduction course can have an extra short (1 hour) introduction on the new microscope, mostly to show the differences and extended possibilities compared to the lsm700. Extra manuals will be provided via our website. Both the LSM700 systems will remain in our imaging facility.
We would like to introduce our new colleague Marla Lavrijsen, she started this april within the OIC.
She will be a new member of our team assisting users of our confocal microscopes during their experiments and introductions.
The NWO Roadmap consortium NL-bioimaging has officially started.
The Erasmus Optical Imaging Centre is one of the imaging nodes within NL-BioImaging, an infrastructure for advanced microscopy within the Netherlands. With the the start of this initiative the OIC receives a five year funding for development of automated microscopy.
