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Course OIC News

OIC course Microscopic Image Analysis

On Monday and Tuesday Jan 29th-30th 2024 the OIC organizes in cooperation with the Erasmus MC graduate school the two-day Image analysis course “Microscopic Image Analysis: From Theory to Practice”. This course focuses on first time users and explains the basis of image analysis and how to use this in Fiji (ImageJ).

Course description 

Microscopic images contain much more information than normally is retrieved. Extracting this information by visual inspection and manual measurement is not only cumbersome but also subjective. Automation of image analysis tasks by using a computer and the right software tools allows for higher efficiency, accuracy, objectivity, reproducibility, and completeness. 

Requirements 

Participants should bring their own laptop to the course for the practical exercises. Software and sample image data will be provided during the course. No prior knowledge of image processing is required. Participants have the opportunity to bring their own image data and directly apply the newly acquired image analysis techniques. 

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Microscope OIC News

Evident FV4000 confocal demonstration

From February 5th to 9th 2024 Evident (formerly Olympus), in association with the OIC, will organize a demonstration of the newly released Fluoview FV4000 point scanning confocal. 

Researchers who are interested to test the possibilities of this new generation laser scanning confocal are invited to bring their own samples for testing. 

The FV4000 can image most of the regular samples used on the current OIC confocals. In addition, the confocal setup should be especially interesting for researchers using near infrared (NIR) fluorescent probes, or more than 4 fluorescent stains. 

Special objectives will be available for imaging organoids and tissues. 
Specifications of the demonstration setup include: 

  • Inverted microscope 
  • 10 laser spanning the wavelength range from 405 nm – to 785 nm 
  • 6 detectors for simulaneous imaging and spectral unmixing 
  • Incubation system for live cell imaging (37°C/5% CO2) using 35mm dishes, multiwell slides and multiwell dishes 
  • Resonant scanner for fast (>20fps) imaging 

If you are interested to test the FV4000, please send us an email with a short description of the sample you would like to image. 

Categories
Microscope OIC News

New microscope installed: Zeiss LSM900

In december the new Zeiss LSM900 is in Be-311 will be available for use. After the installation this summer we have fully tested and optimized the system for our imaging facility.

About the microscope

This microscope is also for fixed specimens, has 3 detectors and has the Zen-blue 3.7 software with an extended tile-scan module.

LSM700 users who did the introduction course can have an extra short (1 hour) introduction on the new microscope, mostly to show the differences and extended possibilities compared to the lsm700. Extra manuals will be provided via our website. Both the LSM700 systems will remain in our imaging facility.

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Imaging Facility OIC News

Our new colleague Marla

We would like to introduce our new colleague Marla Lavrijsen, she started this april within the OIC.

She will be a new member of our team assisting users of our confocal microscopes during their experiments and introductions.

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Imaging Facility OIC News

NWO Roadmap NL-BioImaging started

The NWO Roadmap consortium NL-bioimaging has officially started.

The Erasmus Optical Imaging Centre is one of the imaging nodes within NL-BioImaging, an infrastructure for advanced microscopy within the Netherlands. With the the start of this initiative the OIC receives a five year funding for development of automated microscopy.